# Histology and Histomorphometry Core (HHC)

> **NIH NIH P30** · INDIANA UNIVERSITY INDIANAPOLIS · 2024 · $142,157

## Abstract

PROJECT SUMMARY/ABSTRACT
The Histology and Histomorphometry Core (HHC), Core B, will provide inhouse expertise, techniques, equipment,
and technical innovation to facilitate ICMH investigators from vastly different subspecialties to use histological
approaches in order to assess tissue/cell level mechanisms in their developmental/physiological/disease
models. The HHC will facilitate histological and histomorphometric analyses of bone, muscle, cartilage, ligament,
tendon, intervertebral disk. The HHC will provide consultation on study design and post-study data interpretation
and provide training programs and education in all techniques covered by the Core. Aim 1: Provide histological
processing and quantitative analysis of musculoskeletal tissues. Histological preparation of bone, muscle, cartilage,
ligament and tendon utilizing outcome-specific staining techniques will be provided. Processing techniques
will include undecalcified/decalcified hard tissues, frozen sections, immunohistochemistry, and special staining.
Experienced analytical support for histomorphometric and immunohistometric assessments will also be a Core
service. Innovation will focus on integrating with Core C to develop spatial transcriptomics of musculoskeletal tissue
using histological sections. Aim 2: Provide consistency and comparability of measurement modalities across
studies. The Core will ensure consistency in the protocols among projects and provide quality control standards
for technical procedures. This will contribute to the cost-effectiveness and comparability of measurements across
all projects. Coordinated guidance will be provided to investigators on selection and quality management of appropriate
preclinical and clinical assessments, and centralized assessment of outcomes. Aim 3: Provide consultation
on study design and post-study data interpretation. Core directors and advisors will provide consultation on
study design and post-study interpretation related to any Core analytical service. Services will be made available
to internal investigators outside of musculoskeletal biology who need analysis of musculoskeletal tissues and early
career investigators outside the University who could benefit from services. Aim 4: Provide training and education
in histological analyses. Faculty, fellows, and graduate students will be offered annual training sessions in
analytical methods in research related to histology. A one-day training session will be provided twice each year,
each focused on one of the approaches utilized by the Core; slots will be made available for early career investigators
outside the univeristy. We will also arrange 1 :1 training with laboratory supervisors on request. The Core
will also support technical personnel professional development training to allow them to expand methodologies
incorporated into the Core. By providing consistent methodological support, reproducibility will be improved and
by providing training and advice to investigators, th...

## Key facts

- **NIH application ID:** 10844228
- **Project number:** 1P30AR083854-01
- **Recipient organization:** INDIANA UNIVERSITY INDIANAPOLIS
- **Principal Investigator:** Matthew R Allen
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $142,157
- **Award type:** 1
- **Project period:** 2024-09-02 → 2029-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10844228

## Citation

> US National Institutes of Health, RePORTER application 10844228, Histology and Histomorphometry Core (HHC) (1P30AR083854-01). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10844228. Licensed CC0.

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