# Cytometry and Antibody Technology Facility

> **NIH NIH P30** · UNIVERSITY OF CHICAGO · 2024 · $154,255

## Abstract

PROJECT SUMMARY/ABSTRACT - CYTOMETRY AND ANTIBODY TECHNOLOGY FACILITY
The Cytometry and Antibody Technology (CAT) Facility remains one of the highest utilized core facilities by
UCCCC investigators. CAT supports the development of state-of-the-art flow cytometric assays for cellular
phenotype and function for University of Chicago Medicine Comprehensive Cancer Center (UCCCC)
investigators. The mission of CAT is to provide cutting-edge flow cytometry technology operated by expertly
trained personnel; and provide expert technical consultation and training of researchers on cytometric and
antibody technology. In total, CAT supported 84 unique UCCCC members during this time period, with 188
federally funded peer-reviewed cancer- relevant grants and 105 peer reviewed publications (60% in journals
with impact factor ≥ 10). Specific Core accomplishments during the current funding period include obtaining
a new BD FACs Fortessa for 15 color flow cytometry, an Aria Fusion for sorting human samples for
translational studies, and a new CyTOF Helios mass cytometer that was funded by a successful NIH S10
award.
UCCCC members benefit from this Core through: (1) Reduced costs – Through the CCSG support, UCCCC
members are provided with high-end instrumentation at a reduced cost; (2) Quality control – All the flow
cytometers are monitored on a daily, monthly, and yearly basis to ensure that they perform with a reliable
consistency. For example, calibration beads are run daily to identify drift in laser alignment or detector
performance, and instruments are decontaminated monthly, with laser power measurement and an in-line
sheath filter replacement every six months. The staff also run a descriptive protocol of each instrument to
ascertain quantum efficiency and background noise in each channel, and calibration in selected channels
using MESF beads. Equivalent protocols are performed on the CyTOF, ImageStream and the Bioplex; (3)
Expert Training and Consultation.

## Key facts

- **NIH application ID:** 10845106
- **Project number:** 2P30CA014599-48
- **Recipient organization:** UNIVERSITY OF CHICAGO
- **Principal Investigator:** George Parsons Langan
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $154,255
- **Award type:** 2
- **Project period:** 1997-09-01 → 2029-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10845106

## Citation

> US National Institutes of Health, RePORTER application 10845106, Cytometry and Antibody Technology Facility (2P30CA014599-48). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/10845106. Licensed CC0.

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