# Chemistry/Biochemistry/Pharmacology Core

> **NIH NIH P01** · NORTHEASTERN UNIVERSITY · 2024 · $367,011

## Abstract

RESEARCH & RELATED - OTHER PROJECT INFORMATION - PROJECT SUMMARY/ABSTRACT
This Core B facility will serve as a technical and scientific support unit for the three Projects (1, 2, 3) of this
Program Project Grant. Its major goals involve: (1) the re-synthesis and scale-up of compounds in sufficient
quantities to address the in vitro and in vivo needs of the research laboratories involved in the projects; (2) the
testing of all new orthosteric ligands for their affinities for the CB1 and CB2 cannabinoid receptors; (3) the testing
of orthosteric ligands for their abilities to bind irreversibly/tightly to CB1 and CB2; (4) determining the functional
properties of ligands initially in the cAMP assay (both orthosteric and allosteric); (5) the testing of anandamide
analogs for their abilities to act as substrates of the endocannabinoid deactivating enzyme, FAAH; (6)
determining the metabolic and plasma stabilities of ligands using liver microsomal and plasma preparations,
respectively; (7) screening for off-target interactions against a broad panel of G protein-coupled receptors, ion
channels, transport proteins as well as endocannabinoid metabolizing enzymes; and (8) evaluation of a select
group of novel ligands in mice for their bioavailability in CNS and plasma; the top candidates of each class in an
extensive pharmacokinetic profiling. Compounds produced under the auspices of Core B will also be made
available to other laboratories identified in this Program Project whose collaboration is at no cost to the grant.

## Key facts

- **NIH application ID:** 10846845
- **Project number:** 5P01DA009158-22
- **Recipient organization:** NORTHEASTERN UNIVERSITY
- **Principal Investigator:** Spyros P Nikas
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $367,011
- **Award type:** 5
- **Project period:** 1994-09-30 → 2027-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10846845

## Citation

> US National Institutes of Health, RePORTER application 10846845, Chemistry/Biochemistry/Pharmacology Core (5P01DA009158-22). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10846845. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*