# CSHL Single Cell Analysis Course (2023-2027)

> **NIH NIH R25** · COLD SPRING HARBOR LABORATORY · 2024 · $161,579

## Abstract

ABSTRACT
CSHL Laboratory Course in Single Cell Analysis
The proposed Cold Spring Harbor Laboratory (CSHL) course Single Cell Analysis to be held annually
in July 2023– 2027, is part of the CSHL’s postgraduate training program in cell and molecular biology.
This is a short, intensive 2 week hands-on course which prepares students to enter directly into
research that makes use of advanced and/or specialized molecular techniques in contemporary
aspects of singe cell-based research. General themes to be covered during the course include
quantitative single cell analysis by RNAseq, genomic DNA analysis, proteomics, and metabolomics.
Specific experimental techniques taught in the course include: 1) Droplet- and microwell based single
cell isolation; 2) Single cell RNA seq library preparation; 3) Single molecule FISH; 4) Single cell
peptidomics; 5) Single cell western blot; 6) Photoactivatable single cell probes; 7) Single cell mass
spectrometry; 8) Soft X-ray tomography; 9) Introductory single cell sequencing analysis. Experimental
techniques are taught in the context of the broader conceptual advances that they may provide. The
course invites lecturers who have made significant contributions in their fields to give up-to-the-minute
reports on current research. The trainees are chosen by the course faculty from larger pools of
applicants and range from graduate students to senior investigators and research physicians. Because
of the short duration of this course, senior, as well as junior individuals can attend and receive a short,
intense period of training in an environment remote from other demands on their time and attention.
The course also provides an unusual opportunity for established scientists to apply these advanced
techniques to their own research interests, or to retrain in an important specialty within neuroscience
with which they may not be familiar. Methods and concepts taught in the course are disseminated to
the wider research community through the publication of laboratory manuals and online resources.

## Key facts

- **NIH application ID:** 10847367
- **Project number:** 5R25HD111361-02
- **Recipient organization:** COLD SPRING HARBOR LABORATORY
- **Principal Investigator:** DAVID J. STEWART
- **Activity code:** R25 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $161,579
- **Award type:** 5
- **Project period:** 2023-05-24 → 2028-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10847367

## Citation

> US National Institutes of Health, RePORTER application 10847367, CSHL Single Cell Analysis Course (2023-2027) (5R25HD111361-02). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10847367. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*