ABSTRACT: FLOW CYTOMETRY AND HUMAN IMMUNE MONITORING SR (FCHIMSR) The Flow Cytometry and Human Immune Monitoring Shared Resource (FCHIMSR) provides Sidney Kimmel Cancer Center (SKCC) members with state-of-the-art fluorescence activated cell sorting and analysis capabilities, as well as full service immune profiling for clinical and translational scientists. The SR was founded as the Flow Cytometry Shared Resource (FCSR) in 1991 and has been continuously funded by the CCSG since 1996. Due to complementary expertise and services, the FCSR merged with the Clinical Cancer Immunology Core (CCIC), a developing core performing ongoing assessments of immune function in patients enrolled in clinical trials, in 2022 to become the FCHIMSR. The FCHIMSR maintains state-of-the-art instrumentation, including Bioscience cytometers, Luminex FM3D, and Nanostring GeoMX spatial transcriptomic profiler and provides expertise in high-parameter panel design, data analysis and interpretation, standardized protocols, and technical support. Additionally, the FCHIMSR aids clinical researchers with sample collection, isolation, and storage; cytokine, growth factor, and other soluble analyte profiling; and spatial transcriptomic analysis. The FCHIMSR is led by Director Larry Harshyne, PhD, Associate Professor of Microbiology and Immunology. Harshyne led the CCIC for four years and brings 25 years of experience in flow cytometry and immunology expertise to the SR. Harshyne transitioned into the Director’s role upon the retirement of long-time Director Jianke Zhang, PhD. FCHIMSR is centrally located within the headquarters for the SKCC, the Bluemle Life Sciences Building (BLSB) at TJU, and is readily accessible to SKCC consortium members via a short walk (<1 mile). The Specific Aims of the FCHIMSR are to: 1) Provide high-quality and affordably priced services for analysis and sorting of cells, biological particles, and extracellular vesicles, 2) Provide end-to-end services for clinical investigators wishing to perform immune assays, and 3) Provide education and training to SKCC members, staff, and trainees. During the project period, the FCHIMSR served 114 SKCC members, 59 (51.8%) of which had peer-reviewed funding, representing all four Research Programs. FCHIMSR services were utilized by 155 total users in 117 peer reviewed publications (average impact factor 10.15), 41 grant applications, and 3 clinical trials. In line with the SKCC strategic plan, IMPACT PHL, and under the guidance of the Director and advisory boards, the FCHIMSR has set six goals for future development: 1) Upgrade bench-top analyzers to new multilaser cytometry systems through NIH S10 grants and supplemental institutional funding, 2) Acquire 96-well plate readers and automated sampling stations to increase high-throughput analysis, 3) Disseminate cytometry protocols to standardize staining and strengthen analyses, 4) Expand educational offerings to include monthly working groups and classroom lectures, 5)...