ABSTRACT: TRANSLATIONAL PATHOLOGY SHARED RESOURCE (TPSR) The Sidney Kimmel Cancer Center (SKCC) Translational Pathology Shared Resource (TPSR) provides essential histopathology, molecular pathology, and digital pathology services to members across the consortium, supports novel cancer research through expert pathological analysis, and provides access to fresh, frozen, and formalin-fixed paraffin-embedded (FFPE) tissue from a diverse patient population. TPSR maintains state-of-the-art instrumentation and uses the latest histopathology techniques to conduct visual evaluation of the morphology of human or animal tissues as well as cellular and engineered tissue/organ samples. TPSR facilitates access to tissues banked in a College of American Pathologists (CAP)-accredited biorepository collected from patients with cancer. TPSR provides molecular pathology services such as immunohistochemistry (IHC) and in situ hybridization (ISH), followed by image scanning and analysis. TPSR also offers automatic animal complete blood cell counts (CBC). Established in 2000, the TPSR has been continuously funded by the CCSG since 2001. With more than 20 years of clinical and experimental pathology service experience, TPSR staff counsel, train, and educate SKCC members on histopathology research strategies, experimental design, and techniques to explore, validate, and visualize their research findings. The TPSR is led by Co-Directors Wei Jiang, MD, PhD, and Haifeng Yang, PhD. Jiang is a senior practicing anatomic pathologist who provides clinical and pathological expertise and overall guidance for TPSR. Yang is a funded cancer investigator who oversees the facility’s daily operation and provides scientific consultations to staff and TPSR users. The TPSR is in the Bluemle Life Sciences Building and is easily accessible to all SKCC investigators via a short walk (<1 mile). The Specific Aims of TPSR are to: 1) Provide technical support to enhance all histotechnical aspects of oncology research, 2) Provide expertise and guidance in experimental design, analysis, and technical troubleshooting via strategic and technical consultation, training, and education through a single port, 3) Offer full range, state-of-the-art histopathology services, and 4) Provide access to a CAP-accredited biorepository. During the project period, the TPSR served 84 SKCC members, 74 (88.1%) of which had peer-reviewed funding, representing all four Research Programs. TPSR services were utilized by 128 total users in 142 peer reviewed publications (average impact factor 9.97), 38 grant applications, and 7 clinical trials. In line with the SKCC strategic plan, IMPACT PHL, and under the guidance of the Co-Directors and advisory boards, the TPSR has set three goals for future development: 1) Provide multiplex IHC and IF services to maximize the amount of data acquired from individual samples, 2) Enhance image analysis services through use of the multiplex IHC/ISH staining platform and a multi-spectral...