# timsTOF HT LC-MS System for UR Proteomics Research

> **NIH NIH S10** · UNIVERSITY OF ROCHESTER · 2024 · $1,105,757

## Abstract

Abstract
We are requesting funds for the purchase of a timsTOF HT mass spectrometry (MS) and liquid
chromatography system (Bruker) to be used for qualitative and quantitative proteomic analyses. This
instrument will greatly expand the proteomic capabilities of the University of Rochester Mass Spectrometry
Resource Laboratory (MSRL) and the services it offers to its user-base throughout the University of Rochester
and Western New York. Currently, the existing MS instruments at the MSRL are being used at full capacity
and there is a critical need for additional modernized instrumentation to alleviate project backlogs and take
advantage of recent advances in label-free Data Independent Acquisition (DIA) proteomics. The acquisition
of the timsTOF will augment our aging “3D” mass spectrometer (capable of detection of ions based LC
retention time, mass to charge and MS/MS fingerprint) with a “4D” ion mobility platform capable of additional
separation based on collision cross-section (CCS) properties of ions.
Recent advances in mass spectrometry have provided powerful tools for high-throughput analysis of proteins
that are widely employed in medical and biological research. Proteomic analyses are typically used for
discovery applications and in clinical or analytical screens that require large-scale target detection and
quantification of complex protein mixtures. Due to its potential for high impact on existing NIH-funded research
programs and Centers, the University of Rochester Medical Center (URMC) has targeted proteomics as an
area of critical development. The University's commitment to developing and supporting the MSRL has been
essential for providing accessibility to modern mass spectrometry resources, services and expertise for the
University of Rochester and Western New York research communities. The facility has experienced a
dramatic increase in demand in recent years, spurred in part by an overall shortage of accessibility to high-
end mass spectrometers in the region.
The requested timsTOF HT mass spectrometer is a versatile instrument that excels at high-throughput
quantitative proteomic applications, at a level beyond the capabilities of the current mass spectrometers in
the core facility. Specifically, the timsTOF instrument offers high-capacity Trapped Ion Mobility (TIMS)
analyzer, enables Parallel Accumulation and Serial Fragmentation (PASEF) and accurate, reproducible ion
mobility separation and CCS determination. Importantly, the instrument can achieve sequencing speeds up
to 150 Hz and 60,000 (FWHM) resolution at 1222 m/z. This rate of data acquisition far exceeds our current
instrument and will enable significant enhancements in our project turnaround times. In this application, 9
major users and 4 minor users provide project descriptions that impact at least 20 NIH research grants. The
diversity of users highlights the importance of this technology and the unmet level of need that will be
addressed by the instrument.
The need for ...

## Key facts

- **NIH application ID:** 10848739
- **Project number:** 1S10OD036212-01
- **Recipient organization:** UNIVERSITY OF ROCHESTER
- **Principal Investigator:** SINA GHAEMMAGHAMI
- **Activity code:** S10 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $1,105,757
- **Award type:** 1
- **Project period:** 2024-05-01 → 2025-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10848739

## Citation

> US National Institutes of Health, RePORTER application 10848739, timsTOF HT LC-MS System for UR Proteomics Research (1S10OD036212-01). Retrieved via AI Analytics 2026-07-16 from https://api.ai-analytics.org/grant/nih/10848739. Licensed CC0.

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