# Flow Cytometry Shared Resource

> **NIH NIH P30** · GEORGETOWN UNIVERSITY · 2024 · $69,490

## Abstract

FLOW CYTOMETRY & CELL SORTING SHARED RESOURCE: PROJECT SUMMARY
Flow cytometry is a mainstay technology required to support a dynamic range of basic science and clinical
biomedical research programs across scientific disciplines. The Flow Cytometry & Cell Sorting Shared Resource
(FCSR) provides an array of options for both (1) cell analysis and (2) cell sorting required for functional cellular
analysis or downstream molecular inquiry. Due to the nature of FCSR services, flexible on-site flow cytometry
facilities are vital to maintain the viability of sorted cells and to perform time-sensitive analysis that supported 30
Georgetown Lombardi Comprehensive Cancer Center (LCCC) Members in FY2022. Over the current project
period the FCSR has been transformed into a multi-site Shared Resource (SR), which now provides NJ-based
LCCC Members access to on-site FCSR services. Led by Director Karen Creswell, PhD and Manager Yuanyuan
Tian, PhD, who oversees FCSR operations on the NJ campus, the FCSR support staff (three FTEs) provide flow
cytometry services, experimental and panel design, and post-acquisition analysis. The development of new
methodologies is also prioritized to ensure researchers have access to the most current flow cytometry services.
Creswell and Tian have integrated and standardized FCSR services across the Consortium. The FCSR provides
value to research projects by separating cells based on transfection-based markers, stem cell panels, and
additional cell surface and intracellular markers. Functional assays for cell cycle, apoptotic status, and oxidative
stress are performed in addition to immunophenotyping. Immunophenotyping of mouse and human tissues and
tumor infiltrating lymphocytes (TILs) has improved due to the 16-color capabilities of the BD LSRFortessa and
the 28-color capabilities of the BD FACSymphony A3 cell analyzer. The FCSR receives input from the FCSR
Advisory Committee (AC) and annual user surveys to prioritize development and assess programmatic needs
across the consortium. To this end, new FCSR initiatives aim to provide cutting-edge mass cytometry access to
LCCC Members through the addition of the Standard BioTools CyTOF XT system, greatly expanding user
analysis to more than 40 simultaneous markers, providing an invaluable tool for cancer and immune studies. In
FY2022, the FCSR provided services to 30 LCCC Members across two Research Programs (12 Cancer Cell
Biology [CCB] members, and 18 Cancer Host Interactions [CHI] members). During the current project period,
the FCSR contributed to 52 publications, of these 28 in high impact (IF>10) journals including Nature, Nat
Immunol., and Cell Mol Immunol. The FCSR also provided support that enabled the success of three clinical
trials and 36 extramurally-funded research project grants.

## Key facts

- **NIH application ID:** 10849016
- **Project number:** 2P30CA051008-30
- **Recipient organization:** GEORGETOWN UNIVERSITY
- **Principal Investigator:** KAREN A CRESWELL
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $69,490
- **Award type:** 2
- **Project period:** 1997-08-15 → 2029-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10849016

## Citation

> US National Institutes of Health, RePORTER application 10849016, Flow Cytometry Shared Resource (2P30CA051008-30). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10849016. Licensed CC0.

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