# Microscopy & Imaging Shared Resource

> **NIH NIH P30** · GEORGETOWN UNIVERSITY · 2024 · $43,629

## Abstract

MICROSCOPY & IMAGING SHARED RESOURCE: PROJECT SUMMARY
The Microscopy & Imaging Shared Resource (MISR), established in 1989 and Cancer Center Support Grant
(CCSG)–funded since 1991, supports Georgetown Lombardi Comprehensive Cancer Center (LCCC) Members
apply advanced microscopy and imaging-based technologies to their research. The MISR provides access to
well-maintained imaging systems and the expertise, training, and reagents necessary for investigators to make
optimal use of these technologies on both the DC and NJ campuses. The MISR provides LCCC Members access
to instrumentation for bright-field microscopy, wide-field fluorescence microscopy, and more advanced
applications, including high-speed and time-lapse confocal microscopy, total internal reflection fluorescence
microscopy (TIRF), multispectral emission imaging, fluorescence lifetime imaging (FLIM), Förster resonance
energy transfer (FRET), fluorescence recovery after photo-bleaching (FRAP), and deep tissue multiphoton
imaging. During the current project period, the MISR has added three super-resolution imaging platforms (two
in DC one in NJ), phasor-based fluorescence lifetime imaging, second- and third-harmonic generation imaging,
and has greatly enhanced the high-speed confocal imaging and TIRF capabilities of the facility with the
acquisition of multiple new imaging platforms, both in DC and NJ. In addition to the acquisition and analysis
software on each imaging platform, the MISR maintains four dedicated offline image analysis workstations
running MetaMorph, Volicity, Huygens, and other analytic tools. The MISR, led by Co-Directors Michael Johnson,
PhD, in DC, and Steven Park, BS, in NJ is supported by two additional faculty members and managers in DC
and NJ. The MISR team has considerable experience and expertise with wide-field and confocal optical imaging,
time-resolved fluorescence spectroscopy, multiphoton imaging approaches, molecular cytogenetics, the
maintenance of custom imaging platforms, sample preparation and processing, and a wide array of image
analysis approaches. The MISR receives input from the MISR Advisory Committee and annual user surveys to
prioritize development and assess programmatic needs across the consortium. The MISR provides LCCC
Members with expert advice, training, and assistance with experimental design, data acquisition, and image
analysis, to ensure that they can take optimal advantage of the resources available. Seminars and workshops
on various topics are offered regularly. In FY2022, the MISR provided services to 26 LCCC Members across all
three Research Programs (12 Cancer Cell Biology Members, 13 Cancer Host Interactions Members, and 1
Cancer Prevention and Control Member). During the current project period, MISR contributed to 46 publications,
including 13 papers in high-impact journals, and provided support that enabled the success of 30 extramurally
funded grants. Future plans include the addition of a light-sheet microscopy platform in DC...

## Key facts

- **NIH application ID:** 10849018
- **Project number:** 2P30CA051008-30
- **Recipient organization:** GEORGETOWN UNIVERSITY
- **Principal Investigator:** MICHAEL D JOHNSON
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $43,629
- **Award type:** 2
- **Project period:** 1997-08-15 → 2029-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10849018

## Citation

> US National Institutes of Health, RePORTER application 10849018, Microscopy & Imaging Shared Resource (2P30CA051008-30). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10849018. Licensed CC0.

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