TISSUE CULTURE & BIOBANKING SHARED RESOURCE: PROJECT SUMMARY The Tissue Culture & Biobanking Shared Resource (TCBSR) is an integral part of Georgetown Lombardi Comprehensive Cancer Center (LCCC) research activities and has been funded by the Cancer Center Support Grant (CCSG) since 1993. Bassem R. Haddad, MD, has led the TCBSR as Director since 2002. The TCBSR provides services to support LCCC Members with all tissue culture-related aspects of their work. It also offers unique and necessary services that enable biobanking of blood and other biofluid specimens obtained from subjects enrolled in various LCCC studies. The tissue culture services available are as follows: (1) cell culture and cell banking; (2) conditional reprogramming of cells to establish primary epithelial cell cultures from normal and cancer tissues; (3) Epstein-Barr virus (EBV) B-cell transformation service to facilitate cancer genetics studies; (4) maintaining the LCCC repository of established cell lines; (5) performing quality control tests for cell lines, including cell line authentication and mycoplasma testing services; (6) providing a safe and secure cryostorage facility for investigators to keep frozen stocks of their cancer cell lines and other reagents; (7) testing and supplying tissue culture reagents of the highest quality that have been tested for suitability in the relevant systems, as inexpensively as possible; (8) equipping, monitoring and stocking three separate shared tissue culture laboratories for use by LCCC investigators; and (9) training in the basic principles of tissue culture with hands-on experience. The TCBSR is also responsible for biobanking blood and other biofluid specimens obtained from subjects enrolled in various LCCC studies by the Survey, Recruitment, & Biospecimen Collection Shared Resource (SRBSR) and individual LCCC Members. The biobanking services include the processing of specimens (e.g., preparing aliquots of serum, plasma, buffy coat and peripheral blood mononuclear cells [PBMCs]), banking the specimens, and disbursing as needed. The TCBSR continues to be heavily used by a large number of LCCC Members conducting basic science research, translational research, as well as for some clinical trials (biobanking). In FY2022, the TCBSR provided services to 38 LCCC Members from all three Research Programs: 18 from the Cancer Cell Biology Program, 14 from Cancer Host Interactions Program, and 6 from Cancer Prevention and Control Program. Collaboration between TCBSR and seven other LCCC Shared Resources is an active process, particularly in biobanking where TCBSR works synergistically with the SRBSR to enable the biobanking of all blood and other biofluid specimens obtained from subjects enrolled by SRBSR in various LCCC studies. The capabilities of the TCBSR also provided key tissue culture and biobanking support that enabled the success of 68 extramurally funded research project grants, 8 clinical trials, and 60 peer-reviewed publications, inc...