The goal of this proposal is to purchase a Bruker 15.2T magnet to replace the out-of-date 14T Magnex magnet, which has been dedicated to supporting the high-resolution anatomical and functional dynamic brain and whole-body mapping of animals and ex-vivo human brain tissue imaging. The Martinos Center at the Massachusetts General Hospital has been at the forefront of developing advanced functional mapping methods, e.g., functional MRI, and implementing cutting-edge MRI methods to bridge the basic and translational studies. To pursue the next- generation cutting-edge imaging methodology and prepare for the higher field MRI translational studies, there is an urgent need to improve our ultra-high field preclinical MRI platform for high- resolution animal and ex vivo imaging. In particular, the proposed Bruker Magnet upgrade will boost the translational potentials of the novel methodology, e.g. the single-vessel fMRI, to bridge cellular and microvascular anatomy and functional dynamics from animal to human brains. Also, this proposal will support over 20 research projects funded by NIH, presenting critical translational efforts on the mechanistic studies of brain disorders including Alzheimer’s Disease (AD), cerebrovascular dementia, and brainstem injury-induced coma. etc., as well as for the internal organ imaging of animal models. The highly synergistic and collaborative research projects outlined in our proposal can be summarized in three main themes: 1) Neurovascular dynamic signaling, 2) Cutting-edge neuroimaging methodology, and 3) Multimodal mechanistic signatures of animal disease models. We have established the multi-modal neuroimaging platform to combine high-resolution anatomical and functional MRI imaging with the emerging neuro- techniques, e.g. optogenetics, optical fiber-mediated biosensor recording of Calcium, Glutamate, etc, promoting novel mechanistic understanding of the complexity of brain function. The proposed ultra-high field magnet upgrade will provide key technological improvement to support the performance of the novel brain mapping methods based on state-of-the-art electronics and software design. Therefore, the proposed instrument upgrade would not only accelerate the progress of the listed projects but also facilitate the translation of cutting-edge MR methodologies as a truly multidisciplinary, regional resource for PHS-funded investigators.