# CORE C - Infection and Inflammation Core

> **NIH NIH P01** · SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE · 2024 · $304,200

## Abstract

SUMMARY
The Core C facility supports research addressing the specific aims of the projects to include the planning and
execution of experimental sepsis cohort studies, hematological analyses of whole blood samples, and
measurements of multiple circulating glycoproteins that includes comparative studies of markers of
inflammatory processes during the onset and progression of sepsis. The Core thereby provides support
addressing the central hypothesis of the program, Protein glycosylation and glycoprotein remodeling alter the
coagulopathy and inflammation of sepsis. Core C provides essential experimental and technological
capabilities that include the analysis of experimental and clinical biological samples derived from mouse and
human species including whole blood, serum, plasma, and, when requested, urine. In collaboration with the
projects, and as published, the core has developed an experimental sepsis protocol in the mouse that includes
standardized and reproducible pathogen infection and monitoring with specific thresholds for data inclusion
representing the onset and progression of mouse sepsis in response to five different human bacterial
pathogens and clinical isolates. This approach has enabled comparative studies spanning the specific aims of
each of the three projects while revealing the presence of different pathogenic pathways that provide a degree
of stratification in the pathogenesis of sepsis due to specific bacterial pathogens. These studies will be further
expanded as indicated in the research projects of this application. In addition, the core facility will complete
hematology analyses of mouse blood samples as well as blood from human volunteers and sepsis patients to
include measurements of white and red blood cells, hematocrit values, hemoglobin abundance, and multiple
platelet metrics. Additional analyses when requested will include flow cytometric studies of cell abundance
representing various blood cell lineages including T and B lymphocytes, neutrophils, monocytes, eosinophils,
and basophils. These hematology analyses will be provided to onsite local project researchers and will be
standardized with identical equipment that is used to undertake comparable hematology analyses at the
second site of project research. The core facility will also generate multiplex analyte datasets from serum,
plasma, or urine samples from mouse and human species in the context of health and sepsis. These multiplex
experiments use investigator-selected and customizable assays yielding quantitative measurements of
essential and important cytokines, growth factors, and various other biological indicators of physiological
systems especially those that impinge upon inflammatory processes. Over a thousand proteins can be
selectively measured in various sets of analytes, and many of these proteins are found at low abundance and
cannot be routinely detected and accurately measured using mass spectrometry. The core thereby provides a
means to query low...

## Key facts

- **NIH application ID:** 10849802
- **Project number:** 5P01HL131474-09
- **Recipient organization:** SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
- **Principal Investigator:** JAMEY MARTH
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $304,200
- **Award type:** 5
- **Project period:** 2016-07-15 → 2026-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10849802

## Citation

> US National Institutes of Health, RePORTER application 10849802, CORE C - Infection and Inflammation Core (5P01HL131474-09). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10849802. Licensed CC0.

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