# Core B - Transgenic Animal Genotyping and Phenotyping Core

> **NIH NIH P20** · UNIVERSITY OF NEVADA RENO · 2024 · $341,288

## Abstract

Project Summary
Genetically modified animals are fundamental for biomedical discovery, but breeding, genotyping, and
maintenance of multiple transgenic lines is burdensome for junior investigators and their small laboratories.
Further, most labs lack the specialized equipment and in vivo techniques needed to fully characterize the impact
of genetic modification on essential cardiovascular functions. Therefore, the goal of the present proposal is to
establish a new Transgenic Animal Genotyping and Phenotyping Research Core (Core B) as a centralized facility
to provide these resources to support the research of the COBRE’s Project Leaders and the COBRE
cardiovascular research group. The specific aims of Core B are to (1) establish and operate a centralized facility
for maintaining genetically modified animals used for the investigation of molecular and cellular signal
transduction pathways in the cardiovascular system; (2) establish and operate a facility that provides services
for evaluating the cardiovascular phenotype of genetically modified animals; and (3) advise, train, and provide
technical support for the use and characterization of transgenic laboratory animals. Briefly, Core B will supply
the Project Leaders with access to a wide range of ready-to-use transgenic, knockout, and genetically encoded
biosensor mice useful for the study of molecular and cellular signal transduction within the cardiovascular
system. In addition, Core B will provide Project Leaders with a suite of in vivo techniques for characterization of
the cardiovascular phenotypes of their animal models. These services include telemetric monitoring of blood
pressure, heart rate, hemodynamics, and locomotor activity; echocardiography for evaluating cardiac function;
and indirect calorimetry for measurement of metabolic and behavioral parameters. Successful completion of this
proposal will alleviate the burden of maintaining and characterizing transgenic animal models. As such, Core B
is vital for the rapid progress of the Project Leaders and their successful transition to independence. Furthermore,
establishment of Core B in the greater Nevada area will provide a solid infrastructure and facility for the
development of a highly competitive Cardiovascular Research group.

## Key facts

- **NIH application ID:** 10850203
- **Project number:** 2P20GM130459-06A1
- **Recipient organization:** UNIVERSITY OF NEVADA RENO
- **Principal Investigator:** Yumei Feng Earley
- **Activity code:** P20 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $341,288
- **Award type:** 2
- **Project period:** 2019-01-01 → 2029-02-28

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10850203

## Citation

> US National Institutes of Health, RePORTER application 10850203, Core B - Transgenic Animal Genotyping and Phenotyping Core (2P20GM130459-06A1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10850203. Licensed CC0.

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