Project Summary – High Spatial and Temporal Resolution Imaging (HSTRI) Core (Core C) The goal of the present proposal is to maintain and enhance a High Spatial and Temporal Resolution Imaging Core (HSTRI) research core (Core C) that offers a wide array of state-of-the-art instruments enabling visualization of cells and tissues samples pharmacologically or genetically labeled with fluorescent indicators. With the assistance of expert staff and unrestricted access to these instruments, greatly enhanced the competitiveness of Research Project Leaders (RPLs) in Phase I, and we will provide the same services to RPLs, pipeline investigators, and Pilot Project leaders in Phase II. This facility is specifically designed to meet the needs of our RPLs but will also enhance the overall capacity for biomedical research at the University of Nevada, Reno. Core C will accomplish the following Specific Aims during Phase II of the COBRE: Specific Aim 1: To operate and expand a facility providing state-of-the-art high spatial and temporal resolution imaging methodologies for studying cellular structure, signaling pathways, and function in health and disease. Recent advances in microscopy have produced a new generation of commercially available instruments that enable biomedical researchers to investigate the relationships between structure and function in biological systems with unprecedented spatial and temporal resolution. The main goal of this Specific Aim is to continue making advanced imaging modalities available to COBRE investigators and to the larger UNR community of researchers. Specific Aim 2: To continue advising, training, and informing investigators in the use of the new technologies. Training users in the operation of HSTRI Core equipment and appropriate sample preparation, as well as educating them about the scientific possibilities offered by these technologies, is crucial to maximizing the Core’s impact on research and for achieving long-term self-sustainability. Core personnel will continue to provide appropriate training and supervision of users, manage the logistical challenges associated with accommodating multiple research projects, and offer workshops on novel microscopy and imaging approaches. Specific Aim 3: To expand the userbase and devise an operational plan that will sustain the long-term viability of the Core. Plans were already implemented during Phase I and will continue to be developed to provide support for the Core after the end of the funded period through a business plan of revenues that includes user fees and institutional support. As was the case for Phase I, HSTRI Core services will continue to be offered to investigators directly associated with the COBRE (Research and Pipeline Project Leaders, and Senior COBRE investigators) and non-COBRE investigators at times not reserved by COBRE investigators. Notable in this latter context, many well-funded laboratories at UNR Med and UNR, including non-biological labs in Engineering,...