Cell Isolation and Organ Function (CIOF) Core The central theme of the CardioPulmonary Vascular Biology (CPVB) COBRE is to facilitate high impact vascular biology research and enable investigators to develop insights into basic mechanisms of vasculopathies and translate these findings into prevention or treatment for heart, lung, and vessel diseases. An increased understanding of the mechanisms regulating vascularization and repair may lead to unique approaches to interrupt disease pathogenesis or to promote or enhance cardiopulmonary vascular and vessel restoration. The Cell Isolation and Organ Function (CIOF) core provides a unique skill set and expertise to Rhode Island vascular biologists by providing quality assurance in isolation, characterization, and propagation of vascular derived cells and fibroblasts and cardiopulmonary ex vivo organ function analyses. The centralization of the cell and ex vivo organ isolation, characterization, and function measurements has helped by standardizing technique and reproducibility, and in turn provided investigators the technical expertise, thus, minimizing variability and providing uniformity in data acquisition for CPVB investigators. The services provided by the CIOF Core have permitted the Investigators to focus their efforts on aspects of their research endeavors related to experimental design, execution and interpretation. During Phase I and II, the CIOF core assisted 78 unique users, provided 5366 services, and performed ~$53,000 worth of services in year 9. Further, CIOF provided training for 36 individuals and provided data analyses for 370 requests (despite pandemic shutdown). The specific aims of the CIOF core are to: i) offer effective and reproducible services in cell isolation and ex vivo organ function; ii) acquire, establish, and disseminate technologies and instrument capabilities to provide state of the art research tools for the scientific community; iii) work with IDeA programs in Rhode Island (RI) and other states to foster scientific networks and collaborations by providing technical services to enhance research productivity.; and iv) maintain a robust line of services that meet the research needs of the scientific community. The CIOF core will continue to support and enhance vascular biology research, including for new pilot project investigators and the scientific community, in developing, facilitating, implementing, and performing technical services to impact the research productivity of our IDeA colleagues and collaborators.