Project Summary This is an application for the acquisition of a Bruker NEO console and 1H/19F-detect, 13C,15N- decouple 5 mm EUZ cryoprobe to replace the nearly 20-year-old console and cryoprobe of the existing 700 MHz NMR spectrometer in the shared-use University of Pittsburgh Department of Structural Biology high-field NMR facility. The requested console and cryoprobe will enable reliable acquisition of high-quality high-sensitivity spectra, both on purified biomolecules in solution and labeled biomolecules in living cells. The instrument requested is configured with four independent radiofrequency channels and includes the capability for high sensitivity detection of 19F, an area of increasing emphasis among our user group. The need for the state-of-the-art 700 MHz spectrometer is justified based on the cutting-edge biomedical research, most of which is NIH- funded, conducted by multiple investigators, and cannot be supported by the existing lower field NMR instruments nor the only other high-field instrument dedicated for biomolecular NMR in the greater Pittsburgh area, a nearly 20-year-old 800 MHz spectrometer that is experiencing an increasing number of failures. The proposed state-of-the-art 700 MHz spectrometer will provide capabilities for structural and dynamics analysis of a wide variety of biological systems and will enable the investigators of this proposal to carry out their current NIH-supported research and to take the existing projects to a new level. The spectrometer will also be critical for training PhD students, most of whom receive their training through the joint University of Pittsburgh/Carnegie Mellon University Molecular Biophysics and Structural Biology Graduate Program. Finally, having this resource on campus will permit us to generate new collaborations with institutions in the State of Pennsylvania and beyond, to attract talented new investigators at all ranks to the University of Pittsburgh, and to train the diverse population of graduate and postdoctoral researchers for the biomedical work-force in the interdisciplinary fields of structural biology, biophysics, and biomaterials.