# CORE C- Protein Expression and Purification

> **NIH NIH P01** · CLEVELAND CLINIC LERNER COM-CWRU · 2024 · $309,873

## Abstract

Project Summary/Abstract
 The proposed Animal Models and Tissue Analysis Core (Core C) is an essential component
to the goals of this PPG. To support the research strategies and success of the individual
projects proposed in this PPG application, Core C will serve each of the 3 projects of the PPG
with a variety of animal models of arterial thrombosis, atherosclerosis, venous thrombosis, and
spontaneous and pristane-induced systematic lupus erythematosus (SLE). In addition, it will
provide comprehensive tissue, blood and urine sample analysis for each of the 3 projects.
Furthermore, Core C will train fellows and technical staff of the individual projects to perform
animal studies. Lastly, Core C has access to archival FFPE specimens at the University
Hospitals that is overseen by Dr. Gilmore, a Co-Investigator of Core C, who will ensure that all
IRB requirements for discarded tissue research have been met. Dr. Gilmore will locate
appropriate tissues for the research of the PPG and provide Core C with the necessary clinical
information to facilitate the analyses. The services Core C will provide require specialized
technical skills and equipment. Core C will alleviate the need for each of the investigators to
establish the techniques in their individual laboratories, thereby increasing efficiency, reducing
experimental variation, and providing economy of scale. The main advantages and innovation of
Core C are to provide a dedicated, highly skillful, accessible and cost effective resource for
animal models and tissue analysis.
 The proposed Core C has extensive experience in animal models for cardiovascular
research focusing on various disease models, including thrombosis, atherosclerosis, vasculitis,
stroke, cardiac hypertrophy, analysis and quantitation of mouse tissue and fluids specimens.
The models and analysis have been well-established and published (see Biosketch of Core C
leader). Building on the experience with animal models and tissue analysis, Core C has also
established both spontaneous and pristane-induced SLE models (See Figures 6 and 7 in
Research Plan). Core C will be led by Yunmei Wang PhD, who has extensive experience in
murine models of vascular injury, thrombosis, atherosclerosis, stroke, and cardiac hypertrophy.
In addition, Dr. Wang's over 25-years' research experience working with animals has prepared
her well for any additional expertise and skills involving animals if needed for Core C. Core C
will quickly adopt any innovative animal models once they become available and are determined
appropriate for the purpose of the projects. Furthermore, Dr. Wang has collaborated with all
project leaders of the PPG (see publication in Biosketch).

## Key facts

- **NIH application ID:** 10851891
- **Project number:** 5P01HL154811-04
- **Recipient organization:** CLEVELAND CLINIC LERNER COM-CWRU
- **Principal Investigator:** Yunmei Wang
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $309,873
- **Award type:** 5
- **Project period:** 2021-09-01 → 2026-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10851891

## Citation

> US National Institutes of Health, RePORTER application 10851891, CORE C- Protein Expression and Purification (5P01HL154811-04). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/10851891. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*