# Bruker timsTOF HT Mass Spectrometer for Accelerating Proteomics Research at Rutgers Newark Campus

> **NIH NIH S10** · RUTGERS BIOMEDICAL AND HEALTH SCIENCES · 2024 · $1,077,584

## Abstract

Project Summary/Abstract
This proposal requests funding for the purchase of a Bruker timsTOF HT mass spectrometer (timsHT MS) coupled
with an Evosep One HPLC in order to meet the increasing demand for high-performance proteomics instrumentation
at Rutgers University Biomedical and Health Sciences (RBHS)-Newark Campus. The Center for Advanced
Proteomics Research (CAPR) at RBHS-New Jersey Medical School in Newark will benefit from this instrument,
enhancing service quality. With ever-increasing service needs from NIH-funded researchers, the existing Lumos
Orbitrap MS has been exhausted, resulting in a long delay for large-scale proteomics studies. This wait time impedes
the progress of NIH-funded investigations. Therefore, adding a timsHT MS at CAPR will speed up the research
momentum of Rutgers' NIH-supported researchers.
Experienced CAPR scientists will operate the new timsHT MS to conduct wide-scale proteomics studies for a major
user group of 15 NIH-funded researchers and others. These investigations aim to analyze more samples quickly and
identify more low-abundant proteins, post-translational modifications, and their positional isomers. To achieve
these objectives, a high-end MS that can offer sensitivity, speed, and time-saving technology to distinguish isobaric
peptides and robustness for boosting instrument uptime. The timsHT MS has a unique blend of engineering and
software functionalities that will enable sensitive proteomics studies to meet three unmet needs. Firstly, deep
proteome coverage will be achieved through dual Trapped Ion Mobility Spectrometry (TIMS), which facilitates ~30-
fold time-focusing of low-level peptide ions, and software that is aware of collision cross-section for in-depth
peptide identification and quantification. Secondly, high sensitivity without compromise on speed and resolution
will be achieved through Parallel Accumulation - Serial Fragmentation technology, which facilitates MS/MS
acquisition at 150 Hz, enabling speedy studies of moderate-level samples with short LC gradients. Thirdly,
instrument uptime and robustness will be maximized through an orthogonally positioned ion source to cut
impurities in the mass analyzer, a dual-TIMS funnel to filter contaminating gas, and an improved focus on low-level
peptide ions.
The NIH-funded purchase of this shared timsHT MS will empower CAPR to overcome the time limitations of existing
equipment and offer complementary MS technologies to support RBHS-Newark researchers and those from
neighboring institutions in carrying out their research goals on NIH-funded grants.

## Key facts

- **NIH application ID:** 10853314
- **Project number:** 1S10OD034300-01A1
- **Recipient organization:** RUTGERS BIOMEDICAL AND HEALTH SCIENCES
- **Principal Investigator:** Hong Li
- **Activity code:** S10 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $1,077,584
- **Award type:** 1
- **Project period:** 2024-09-01 → 2025-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10853314

## Citation

> US National Institutes of Health, RePORTER application 10853314, Bruker timsTOF HT Mass Spectrometer for Accelerating Proteomics Research at Rutgers Newark Campus (1S10OD034300-01A1). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10853314. Licensed CC0.

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