A 400 MHz NMR Spectrometer The Analytical Resources Core (ARC) at Colorado State University is requesting funds to purchase a new 400 MHz NMR spectrometer to expand the capacity of our NMR facility for routine 1D and 2D NMR data collection by undergraduate and graduate student researchers and postdoctoral associates in the chemical sciences. The current NMR systems in the ARC can no longer support the growing number of researchers who need daily access to 400 MHz NMR due to recent growth in research programs and new faculty hires in synthetic chemistry all of whom will rely heavily on NMR to operate their research programs. The instrument will be managed within the ARC that is a Colorado State University institutional shared use Core facility homed in the Office of the Vice President for Research. The instrument will be located in the ARC’s NMR laboratory in the Chemistry Research Building, home to all the synthetic chemistry research groups at CSU. At the present time, we do the greatest portion of our routine NMR work on two walkup 400 MHz Bruker NMR systems. One of these lacks automation limiting high throughput sample analysis. The other system is oversubscribed with usage in excess of accessible user time. The proposed 400 MHz NMR will support full automation and be equipped with a sample changer and an automated high performance broadband probe. The magnet solenoid will be actively shielded, have a long hold helium Dewar and will be fully equipped with a helium recovery system. Recovered helium will be purified and reliquefied on the ARC’s helium liquification system making this spectrometer a highly efficient and sustainable system in the current uncertain helium supply environment. The combination of magnet field control, console technology, broadband probe, sample changer and advanced software will provide required sensitivity, resolution and performance to the NIH-supported research at CSU. The new 400 NMR will serve NIH and other funded projects involved with synthesis, catalysis, chemical biology, medicinal chemistry, sustainable polymer chemistry, nanoparticle design for biological imaging and many others. There are five NIH projects supported by this proposal and a similar number of projects funded by other agencies or CSU new faculty startup funds.