# NovaSeq X Plus Sequencing System

> **NIH NIH S10** · UNIVERSITY OF KANSAS MEDICAL CENTER · 2024 · $1,078,438

## Abstract

Project Summary / Abstract
 The University of Kansas Medical Center (KUMC) Genomics Core (GC) is requesting funds to upgrade
high-throughput genomic sequencing capabilities with the purchase of an Illumina NovaSeq X Plus Sequencing
System (NSXP). The GC has provided genomics services since 2002, initially using GeneChip microarrays,
transitioning to Next Generation Sequencing (NGS) in 2011 with the HiSeq 2000/2500, and advancing to a
NovaSeq 6000 (NS6K) in 2017. Demand for our NGS services has grown continuously, and we now serve a
broad user base at the University of Kansas, affiliated Kansas Institutions, the Central Midwest region, and
multiple states across the U.S. Recent advances in Illumina sequencing technology can reduce user cost,
improve sequence-by-synthesis (SBS) chemistry, increase sequence throughput and data quality, improve
onboard data analysis, and enhance file compression for more efficient data management. Unfortunately, our
current instrument is unable to take advantage of these key advances, thereby disadvantaging our investigators
with higher cost and lower sequencing quality. The proposed upgrade to the NSXP will ensure that GC
sequencing capabilities are, and will remain, optimal for the foreseeable future. In addition to regional and
national investigators representing 45 institutions across 27 states who have sought our services, the upgrade
will have significant impact for our 20 major and 5 minor users with 45 NIH grants (28 R01-equivalent) identified
in this application. Beyond aiding independent research programs, the enhanced attributes of the NSXP will
advance the research missions of multiple NIH-funded centers including the NCI-designated Comprehensive
University of Kansas Cancer Center (P30), the NIA–funded Alzheimer's Disease Research Center (P30); a
NIDDK Center for Polycystic Kidney Disease translational research (U54); an NCATS CTSA (Frontiers: The
Heartland Institute for Clinical and Translational Research (U54): and 3 Centers of Biomedical Research
Excellence (COBRE) funded by NIH P20 Institutional Development Award (IDeA) grants. The NSXP will be
located in space already optimized for Illumina NGS operations, which includes 10x Genomics Chromium X
single cell and MissionBio Tapestri single cell DNA systems, 10x Visium spatial genomics, and a forthcoming
Tecan DreamPrep NGS library automation robotics system. The NSXP will be integrated into a core network
providing comprehensive administrative infrastructure including online request tracking, automated billing, and
user-polling for quality assurance, in addition to bioinformatics support and technical support coupled to a newly
expanded IT infrastructure. The GC is backed by a strong institutional commitment for both the acquisition and
operation of the instrument. The acquisition of the NSXP will enhance rate and quality of discovery while
reducing costs by approximately 59% per gigabase sequenced, thereby providing our large and diverse
investigator c...

## Key facts

- **NIH application ID:** 10853870
- **Project number:** 1S10OD036343-01
- **Recipient organization:** UNIVERSITY OF KANSAS MEDICAL CENTER
- **Principal Investigator:** Peter G Smith
- **Activity code:** S10 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $1,078,438
- **Award type:** 1
- **Project period:** 2024-05-15 → 2025-05-15

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10853870

## Citation

> US National Institutes of Health, RePORTER application 10853870, NovaSeq X Plus Sequencing System (1S10OD036343-01). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10853870. Licensed CC0.

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