# Lightsheet microscope system for the Indiana Center for Biological Microscopy

> **NIH NIH S10** · INDIANA UNIVERSITY INDIANAPOLIS · 2024 · $562,000

## Abstract

Lightsheet microscopy is a technology that provides the ability to rapidly acquire millimeter-scale fluorescence
image volumes at subcellular resolution, providing a practical solution to the challenge of fluorescence
microscopy of large optically-cleared tissue samples. This is a proposal for funding to purchase an Alpenglow
3Di Open-Top-Lightsheet microscope system that would be installed, maintained and managed by the Indiana
Center for Biological Microscopy, a core facility of the Indiana University School of Medicine, the Indiana
University NIDDK U54 George M. O'Brien Center for Advanced Renal Microscopy and Molecular Imaging, the
NIDDK-P30 Indiana Diabetes Research Center and the NIDDK-U54 Indiana Cooperative Center of Excellence
in Hematology. The proposed system will be the first and only lightsheet microscope system available to all IU
School of Medicine investigators, thus fulfilling a currently unmet need to support large-scale, high-resolution
microscopy. The particular system has been chosen based upon an “open-top” design that is uniquely
compatible with the slide-mounted samples used by the researchers described in the proposal, and a novel
hybrid optical path that supports both high-speed, low resolution imaging as well as diffraction-limited high-
resolution imaging. The system will be supported by matching funds obtained from the Department of
Medicine and the Division of Nephrology that will be used to obtain additional equipment and support a
technician to promote productive utilization of the system by users described in the proposal as well as future
IU investigators. The new system will enable project investigators to conduct exciting and entirely new studies
that will enhance their NIH-funded research and advance their goals to address serious issues in human
health.

## Key facts

- **NIH application ID:** 10854318
- **Project number:** 1S10OD036391-01
- **Recipient organization:** INDIANA UNIVERSITY INDIANAPOLIS
- **Principal Investigator:** KENNETH W DUNN
- **Activity code:** S10 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $562,000
- **Award type:** 1
- **Project period:** 2024-04-01 → 2025-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10854318

## Citation

> US National Institutes of Health, RePORTER application 10854318, Lightsheet microscope system for the Indiana Center for Biological Microscopy (1S10OD036391-01). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10854318. Licensed CC0.

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