Project Summary The goal of this U19 Genomic Center for Infectious Disease (GCID) application is to use state-of-the-art model systems to address gaps in knowledge surrounding the genomics of multiple NIAID Category A-C bacterial, viral, fungal, and parasitic mucosal pathogens. The Organoid and Minibioreactor Array Cultivation (OMAC) core will assist in achieving this goal by (1) generating, maintaining, and providing experimentally ready and customized intestinal and airway organoid cultures for the individual projects, (2) providing complex microbial communities from the intestine and airway customized for use by individual projects in experiments exploring how the microbiome affects pathogenesis, and (3) developing new tools, models, and platforms for use by the individual projects to explore additional aspects of intestinal and airway biology that may affect infectious disease pathogenesis. The centralization of these activities in the OMAC core allows the provision of critical biologically relevant human organotypic and bacterial cultures that are essential for the success of the project and ensures standardization and reproducibility. These reagents will facilitate the translation of genomic analysis by validation of key outcomes. Centralization will also result in a reduction in labor, supplies, and equipment costs ultimately resulting in time and cost savings for the overall center. Due to the use of the core by all Center projects, the core stands as a central unifying element that connects the projects. As a result, the OMAC core will enhance interproject communication and enable the comparison of center wide results. The establishment of the OMAC core will be an essential aspect of the success of the Center.