An advanced, high-throughput imaging system for the BNI Structural and Functional Imaging Core

NIH RePORTER · NIH · S10 · $272,744 · view on reporter.nih.gov ↗

Abstract

This application is a request for funds to acquire a Zeiss Axioscan 7 slide scanner for the Structural and Functional Imaging Core at the Burke Neurological Institute. The Institute, dedicated to finding cures for chronic neurological disabilities, requires an automated image acquisition system to advance their NIH-funded research. The mandate of the Imaging Core facility is to provide access to a range of light microscopy systems and provide technical assistance with image acquisition, processing, and analysis to all research groups within BNI. While the Institute has invested in high-resolution confocal and multiphoton microscopy systems, it lacks modern epifluorescence and brightfield imaging systems. Existing microscopy solutions are aging and lack critical features found in the proposed Axioscan 7 system. This scarcity of resources poses a significant bottleneck for the diverse NIH-funded research programs at the Institute. The Axioscan 7 slide scanner, with its automated full slide workflow, unlimited z-stack acquisition parameters, and advanced deconvolution algorithms, offers an optimal combination of features for large area tissue scanning and stitching. The proposed system will greatly expedite research progress by reducing active user time during image acquisition. Furthermore, the surrounding area lacks other automated, high-throughput microscopy resources accessible to BNI investigators, making the Axioscan 7 an indispensable asset. Its implementation will not only benefit currently NIH-funded research programs but also catalyze the progress of studies that are yet to reach the maturity required for NIH funding. The proposed research projects underscore the shared need for an advanced slide scanning system. In conclusion, the extensive capabilities and efficient workflow of the Axioscan 7 will exert a sustained, powerful influence on the conduct of NIH-funded research programs at the Burke Neurological Institute and support breakthroughs in the understanding and treatment of chronic neurological disabilities.

Key facts

NIH application ID
10864309
Project number
1S10OD036432-01
Recipient
WINIFRED MASTERSON BURKE MED RES INST
Principal Investigator
Edmund R Hollis
Activity code
S10
Funding institute
NIH
Fiscal year
2024
Award amount
$272,744
Award type
1
Project period
2024-05-15 → 2025-05-14