# Three-dimensional One-step Aptamer Selection in the Non-fouling Macroporous Hydrogel

> **NIH NIH R01** · PENNSYLVANIA STATE UNIVERSITY, THE · 2024 · $482,777

## Abstract

Project Summary
Nucleic acid aptamers (aptamers) have been used as therapeutic agents or delivery ligands for various
biomedical applications such as regenerative medicine, cancer therapy, molecular biosensing, cell delivery,
detoxification, anti-inflammation, anti-coagulation, etc. However, the success of these applications largely
depends on the discovery and delivery of high-affinity and high-specificity aptamers, which remains challenging
today. Therefore, the objective of this project is to study an innovative method for aptamer selection and
truncation, and to further examine the efficiency of in vivo aptamer delivery in regulating inflammation. We will
work on four specific aims to achieve the objective. In the first three aims, we will focus on aptamer selection
and truncation. Notably, while these three aims are related to one another, the proposed research activities in
these aims can be conducted independently. After the selection and truncation of high-quality aptamers, we will
examine microneedle-mediated aptamer delivery for local, defined treatment of inflammation using an animal
model. The success of this project will not only lead to the development of a technological platform for
therapeutic aptamer discovery, but also pave a new way of fighting against inflammatory cytokines in the
treatment of complicated immune disorders.

## Key facts

- **NIH application ID:** 10867669
- **Project number:** 1R01GM154105-01
- **Recipient organization:** PENNSYLVANIA STATE UNIVERSITY, THE
- **Principal Investigator:** Yong Wang
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $482,777
- **Award type:** 1
- **Project period:** 2024-09-01 → 2028-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10867669

## Citation

> US National Institutes of Health, RePORTER application 10867669, Three-dimensional One-step Aptamer Selection in the Non-fouling Macroporous Hydrogel (1R01GM154105-01). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10867669. Licensed CC0.

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