# Dynamics of Endomembrane Docking and Fusion

> **NIH NIH R01** · UNIVERSITY OF WASHINGTON · 2024 · $373,390

## Abstract

Abstract Summary
COPII mediated ER-to-golgi membrane traffic was first reconstituted using crude cell extracts thirty years
ago. Twenty years ago, the formation of COPII vesicles was recapitulated using pure proteins and lipids.
However, the consumption of COPII vesicles – their uncoating, tethering, docking, and fusion with accep-
tor compartments, is still poorly understood. This is in large measure because the crude transport assay
developed thirty years ago has not been supplanted by a system employing chemically defined components.
In this Project we introduce such a system and use it to test hypotheses about the sequence of biochemical
subreactions in COPII vesicle traffic, the components that execute these subreactions, and the mechanisms
through which they function.

## Key facts

- **NIH application ID:** 10868432
- **Project number:** 5R01GM077349-17
- **Recipient organization:** UNIVERSITY OF WASHINGTON
- **Principal Investigator:** Alexey Jarrell Merz
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $373,390
- **Award type:** 5
- **Project period:** 2006-03-01 → 2025-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10868432

## Citation

> US National Institutes of Health, RePORTER application 10868432, Dynamics of Endomembrane Docking and Fusion (5R01GM077349-17). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10868432. Licensed CC0.

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