# The BCM Knockout Mouse Production and Phenotyping Project (BCM KOMP2)

> **NIH NIH UM1** · BAYLOR COLLEGE OF MEDICINE · 2024 · $2,303,000

## Abstract

ABSTRACT
The goal of the International Mouse Phenotyping Consortium (IMPC) is to develop a resource of null allele lines
for every protein-coding gene in the mouse genome and, through standardized, broad-based phenotyping,
catalog the biological function of each targeted gene. The NIH-funded Knockout Mouse Phenotyping Project
(KOMP2) has supported two previous phases of null allele production and phenotyping, contributing to more
than half of the null allele lines made and characterized by the IMPC. This application describes our plan for
Baylor College of Medicine (BCM) to continue as a KOMP2 site during a third phase of funding. We propose to
produce, cryopreserve, and phenotype null allele mouse lines for 600 protein coding genes. We have developed
a bioinformatics approach to identify and prioritize genes with known or putative human disease associations
but little-to-no in vivo functional annotation for null allele mouse line production. We will generate and validate
null alleles using established CRISPR genome editing approaches and quality control procedures, cryopreserve
all null allele lines using accepted techniques, and deliver germplasm to the MMRRC repositories for community-
wide distribution. These null allele lines represent a gold standard resource for the scientific community and an
important foundation for future research and translational studies. We will utilize our established pipelines to
perform broad-based, standardized adult phenotyping on all mutant lines and assess homozygous lethal and
subviable lines in an embryonic phenotyping pipeline. The phenotyping data we generate will provide
fundamental new insights into mammalian biology as well as the genetic bases of human disease. All allele and
phenotype data will be submitted in real time to the Data Coordination Center, ensuring the rapid dissemination
of all BCM data to the wider biomedical scientific community. We will continue our production and phenotyping
technology development programs, developing our own new approaches and adopting new methodologies from
the community as they arise. The BCM KOMP2 team has the established expertise, experience, and resources
to meet the proposed phase 3 goals in an efficient and cost-effective manner.

## Key facts

- **NIH application ID:** 10868499
- **Project number:** 5UM1HG006348-13
- **Recipient organization:** BAYLOR COLLEGE OF MEDICINE
- **Principal Investigator:** Jason D. Heaney
- **Activity code:** UM1 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $2,303,000
- **Award type:** 5
- **Project period:** 2011-09-16 → 2027-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10868499

## Citation

> US National Institutes of Health, RePORTER application 10868499, The BCM Knockout Mouse Production and Phenotyping Project (BCM KOMP2) (5UM1HG006348-13). Retrieved via AI Analytics 2026-05-27 from https://api.ai-analytics.org/grant/nih/10868499. Licensed CC0.

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