Initially funded as a Clinical and Translational Science Award (CTSA) in 2010, the Georgetown-Howard Universities Center for Clinical and Translational Science (GHUCCTS) is a unique partnership of Georgetown University, Howard University, MedStar Health Research Institute, the Washington DC Veterans Administration Medical Center, and Oak Ridge National Laboratory. Each partner brings unique and complementary strengths to our CTSA Hub. The overall vision of GHUCCTS is to establish, nurture, and grow a transformative translational science (TS) ecosystem wherein scientific excellence and innovation catalyze measurable health improvements via collaborative, multidisciplinary, multi-institutional and community partnerships. We will implement our vision and move forward as a fully mature CTSA with the capacity to serve as a field-leading center for clinical translational science (CTS) innovation while participating in multi-hub collaborations both regionally and nationally. The principles upon which our Specific Aims are based have remained the bedrock of our CTSA since its inception, but have significantly evolved in this application to reflect new, timely CTS priorities and plans, innovations, and knowledge aligned with the current priorities of the National Center for Advancing Translational Sciences’(NCATS) and the National Institutes of Health (NIH). This application describes how we will innovate beyond our current successes and lessons learned in order to grow CTS in the next phase of GHUCCTS, and the specific strategies for accomplishing each of our Specific Aims.