# Mechanisms required to compartmentalize the stem cell niche during organogenesis.

> **NIH NIH R15** · EAST CAROLINA UNIVERSITY · 2024 · $453,000

## Abstract

Project Summary/Abstract:
Stem cells are required for tissue homeostasis and regeneration. Accomplishing these tasks often requires
intimate association between stem cells and their niche, where stem cells efficiently receive critical niche-
derived signals within specialized tissue compartments. These niches are formed by cells that often must
migrate during organogenesis to attain their appropriate position, yet how this morphogenesis is achieved is
unclear. The broad objective of this application is to define – using powerful genetics and incisive optogenetic
approaches in an accessible undergraduate model system – the molecular mechanisms dictating embryonic
establishment of the Drosophila male testis niche. Results from this work will reveal the cellular dynamics and
molecular mechanisms required to assemble a functional model stem cell niche, which are likely to be
applicable to the numerous other stem-cell based tissues. Our previous work showed that Slit and FGF signals
from adjacent visceral muscle are required to assemble the testis niche. In response to these signals, niche
cells express the transcription factor islet, which is required for F-actin polarization and movement to the gonad
anterior. In Aim 1, we will examine key signaling intermediaries required to induce islet expression in response
to gonad extrinsic signaling. In Aim 2, we will define downstream mechanisms that mediate islet function and
pursue predicted islet targets expressed in niche cells. Using an optogenetic approach during in vivo live
imaging, we will also investigate niche cell behaviors reliant upon mechanisms regulating the F-actin
cytoskeleton. In Aim 3, we will identify how niche-extrinsic signaling from another tissue within the gonad
positions the forming niche. Concepts we elucidate will have broad applicability to studying normal
development, promoting success of stem cell replacement therapies, and revealing mechanisms by which
cancer cells often shape their unique tumor microenvironments. Furthermore, funding from this proposal will
support infrastructure at a large regional public university where we will employ a tractable model system that
provides high-impact biomedical research experiences to underserved undergraduates in a supportive training
environment.

## Key facts

- **NIH application ID:** 10873387
- **Project number:** 1R15GM154246-01
- **Recipient organization:** EAST CAROLINA UNIVERSITY
- **Principal Investigator:** Lauren M. Anllo
- **Activity code:** R15 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $453,000
- **Award type:** 1
- **Project period:** 2024-09-01 → 2027-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10873387

## Citation

> US National Institutes of Health, RePORTER application 10873387, Mechanisms required to compartmentalize the stem cell niche during organogenesis. (1R15GM154246-01). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10873387. Licensed CC0.

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