Alcoholic liver disease (ALD) remains the most common chronic liver disease worldwide. Despite extensive studies, no FDA-approved therapy is available for any stage of ALD due to the limited understanding of disease pathogenesis. Therefore, the unmet need to identify novel targets for developing effective therapeutics against ALD is urgent. Alcohol not only alters hepatic lipid and bile acid metabolism but also disrupts the gut microbiome and intestinal barrier function, which results in a leaky gut and bacterial translocation as well as activation of systemic and hepatic inflammation. We have previously reported that conjugated bile acids activate sphingosine- 1 phosphate receptor 2 (S1PR2), which further activates ERK1/2 and AKT. S1P is one of the most studied sphingolipids and is synthesized from sphingosine by either sphingosine kinase 1 (SphK1) or SphK2. S1P can regulate various fundamental cellular responses either as an intracellular signaling molecule or a ligand for five GPCRs, S1PR1-5. SphK2-generated nuclear S1P is a potent natural inhibitor of histone deacetylases (HDAC1/2). Activation of the stimulator of interferon genes (STING) also has been identified as critical signaling in ALD. A recent study reported that SphK2-mediated production of nuclear S1P in CD11b+ macrophages is a strong inhibitor of STING and suppresses the inflammatory response in alveolar macrophages. We have reported that SphK2-/- mice developed more severe fatty liver and hepatic injury in the NIAAA ALD mouse model. Hepatic SphK2 expression levels were markedly reduced in both ALD cirrhotic patients and ALD mouse models. Alcohol-feeding significantly increased intestinal permeability and bacterial translocation in SphK2-/- mice. Our preliminary data further showed that 1) alcohol-feeding induced more severe liver injury in the global SphK2-/- mice than hepatocyte-specific SphK2 knockout (SphK2Hep-/-); 2) intestinal epithelial cell-specific SphK2 knockout (SphK2IEC-/-) mice were more prone to alcohol-induced liver injury compared to SphK2fl/fl mice; 3) RNAseq analysis showed that chronic alcohol feeding significantly disrupted hepatic sphingolipid, fatty acid, and bile acid metabolism and activated inflammatory and fibrotic responses; 4) deletion of SphK2 inhibited the growth of intestinal organoids. Based on these key findings, we HYPOTHESIZE that disruption of SphK2/S1P-mediated signaling pathways in the gut-liver axis plays a critical role in alcohol-induced liver injury. Two specific aims are proposed to test the hypothesis. 1) To investigate the role and mechanisms of SphK2 in modulating hepatic lipid metabolism and inflammatory response under the conditions of acute and chronic alcohol-induced stress. 2): To define the role of SphK2 in modulating intestinal barrier function and to identify the cellular mechanisms by which SphK2 modulates the gut liver axis in response to alcohol-induced stress. Accomplishing these specific aims will significantly advance our ...