PROJECT SUMMARY Family separations due to disasters, armed conflict, and migration affect millions, are expected to increase, and damage children’s health across the lifecourse. Given the extreme trauma of separations, reunifications should occur quickly in the child’s best interest, when safe to do so. Young, pre-verbal children are unlikely to be able to recount contact information of family members. DNA data can be powerful both for identification and for kinship verification of children separated from their families, as DNA can quickly, accurately, and inexpensively provide concrete evidence of genetic family ties. We propose to investigate strategies for (1) protection of children’s DNA data; and (2) consent for children at varying developmental stages to inform development of a U.S. family reunification DNA database strategy to serve as a scalable humanitarian “techquity” intervention to be activated when needed to reconnect separated children with their families. Our goal is to inform U.S.-relevant protocols, partnerships, and infrastructure for DNA-based family reunification responses that can be activated following separations, including after a mass separation event such as a natural disaster, while ensuring protections of children’s DNA data are in place. In the past, DNA data have been successfully used for disaster victim identification and for limited, scenario-based family reunifications of “disappeared” children abducted in war; however, no protocol exists for use of living children’s DNA to reunify families. We can learn from past contexts to develop strategies for securing sensitive samples and data from secondary misuse. We aim to define the scope of current and prior humanitarian use of children’s DNA data to analyze: (a) the contexts in which children’s DNA data are or have been used; (b) the role of DNA in each context; and (c) the extent to which DNA data advances or harms children’s interests. We will interview key informants to capture nuanced scenarios within each context to record factors related to risks and benefits of children’s DNA data use, and apply framework analyses to describe utilities and pitfalls of children’s DNA data use. We also will identify and interview key allies and experts with expertise on age-appropriate consent processes. Interview data will provide insight into principles of consenting children for DNA use for reunification across developmental stages. Finally, we will host a one-day virtual summit among allies and experts that will involve facilitated dialogue and visual mapping to identify and deliberate the contentious matters in consenting for children’s DNA use for reunification. Based on the summit discussions, we will formulate and visualize a set of informed consent principles for children’s DNA use for family reunification. Findings will have immediate impact for informing protocols and policy, and can prompt further research, including the immediate next step of conducting a modified-D...