# Redox Molecular Signaling Core

> **NIH NIH P20** · LOUISIANA STATE UNIV HSC SHREVEPORT · 2024 · $399,854

## Abstract

PROJECT ABSTRACT
The goals of the COBRE Center for Redox Biology and Cardiovascular Disease are to establish a nationally
recognized advanced research and training program in redox biology regulation of cardiovascular disease and
to facilitate the development of junior faculty research programs to achieve major independent research funding.
The Redox Molecular Signaling Core (Core C) provides COBRE investigators and trainees with access to
modern equipment for the detection and quantification of reactive oxygen species, reactive nitrogen species,
and reactive sulfur species. Additionally, this facility enables the analysis of redox-dependent control of protein
function through redox proteomics and metabolomics, provides a centralized facility for cell culture hypoxia
studies, and provides expertise for establishing cell culture models of redox signaling. This range of analytical
techniques to assess redox signaling will be unmatched for a single facility in Louisiana. The functions of the
Redox Molecular Signaling Core are accomplished through two distinct sub-cores, the Analytical Redox Biology
Sub-Core and the Molecular Signaling Sub-Core. The Analytical Redox Biology Sub-Core utilizes equipment for
high performance liquid chromatography (HPLC), specialized chemiluminescent detectors, and mass
spectrometry (MS) to provide high quality, quantitative redox measurements at a centralized facility with
dedicated technical staff. During Phase I, the institution purchased an Orbitrap Exploris 480 mass spectrometer
that significantly expanded our capability to perform proteomic analysis of cell and tissue function in models of
redox signaling. During Phase II, we will expand the functionality of this core through the purchase of new
equipment for reactive sulfur species quantification and additional mass spectrometry equipment to enhance
sensitivity. The Molecular Signaling Sub-Core offers a centralized facility for providing assistance with molecular
biology, for establishing cell culture models of redox signaling, and for affording access to vital equipment for
modeling hypoxic cell injury in culture and for protein analysis using high throughput, automated capillary
electrophoresis. In Phase II, we will mature and expand the Molecular Signaling Sub-Core by adding additional
core services based on the needs of the current COBRE faculty, such as improved methods for transient
transfection of primary cells and enhanced access to next generation sequencing technologies. The faculty and
staff of the Redox Molecular Signaling Core work closely with the COBRE investigators to optimize experimental
design, perform analytical measurements, provide training on the use of core equipment, and assist with data
collection and interpretation. These services provide an invaluable benefit to the COBRE investigators to
advance their individual projects while enhancing the infrastructure of local redox-biology research.

## Key facts

- **NIH application ID:** 10879015
- **Project number:** 5P20GM121307-07
- **Recipient organization:** LOUISIANA STATE UNIV HSC SHREVEPORT
- **Principal Investigator:** Anthony Wayne Orr
- **Activity code:** P20 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $399,854
- **Award type:** 5
- **Project period:** 2023-08-01 → 2028-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10879015

## Citation

> US National Institutes of Health, RePORTER application 10879015, Redox Molecular Signaling Core (5P20GM121307-07). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10879015. Licensed CC0.

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