PROJECT ABSTRACT High-quality biospecimens are critical to cancer research. The CHTN Southern Division leverages the CAP- accredited biorepository broad-consent framework and faculty/staff expertise of the Duke BioRepository & Precision Pathology Center (BRPC) to provide high-quality biospecimens to investigators through the National Cancer Institute’s Cooperative Human Tissue Network (CHTN). Distribution of high quality biospecimens and data and the additional CHTN missions of biobanking best practice dissemination and community education are addressed through the following four specific aims. Aim 1: Efficiently provide high-quality tissue, blood and fluid biospecimens to CHTN investigators for basic and early translational research. Specimen collection is facilitated at Duke University Hospital and Duke networked hospitals Duke Regional Hospital and Duke Health Raleigh Hospital, as well as the CHTN Southern Division subsite at Atlantic Health System in Morristown, New Jersey. The latest technologies in sample processing including viable specimen preservation, tumor enrichment, nucleic acid extraction, genotyping, biomarker phenotyping, whole slide imaging, tissue microarray, immunohistochemistry, digital image analysis and digital spatial profiling are also available. Aim 2: Provide consultation. Duke pathologists and biorepository staff provide expert consultation to CHTN investigators on use of human biospecimens, assay selection and sample processing with an eye toward fit- for-purpose pre-analytics. Aim 3: Develop and disseminate knowledge on high-quality practices for biorepositories. This CHTN Southern Division team will take on new projects and disseminate results through publication and presentation at national and international meetings. The Southern Division PI will continue to serve in national and international leadership roles within the biorepository community. Aim 4: Educate the local, regional and national community on the importance of biospecimens in biomedical research. Locally, the CHTN Southern Division at Duke University will continue to engage the Durham, NC community through the Duke Cancer Institute’s Community Outreach & Engagement Core, participating in two-way dialogue through events such as Community-Scientist Roundtables and Biobank tours, seeking specifically increasing the number of samples available for research from underrepresented populations. The Southern Division will continue to leverage opportunities to educate the public and the scientific community about the importance of having sufficient, high-quality, annotated biospecimens available for research. Examples include podcasts, meeting presentations geared toward general scientific research audiences, newsletters, and social media postings.