# Mass Spectrometry/Proteomics Core

> **NIH NIH P30** · VANDERBILT UNIVERSITY MEDICAL CENTER · 2024 · $79,182

## Abstract

PROJECT SUMMARY: VDDRC MASS SPECTROMETRY (MS) AND PROTEOMICS CORE
The MS/Proteomics Core provides cost-effective, state-of-the-art instrumentation and expertise to
investigators in the Vanderbilt Digestive Disease Research Center (VDDRC). This core leverages the
expert personnel and cutting edge instrument facilities located in the Vanderbilt Mass Spectrometry
Research Center to provide specialized services to VDDRC members. This core will be used for
identification and quantification of small molecules, identification, characterization, and quantification of
proteins, and biomolecular tissue imaging and profiling. Fifty-eight VDDRC investigators have used the
core extensively during the current funding period for digestive disease-related projects leading to 73 peer-
reviewed original publications. Services used include: analytical method development, assistance with
experimental design, inductively coupled MS, multiplex quantitative proteomics with isotope tagging
methods, phosphoproteome characterization, identification of post-translational modifications, elemental
analysis by ICP-MS, molecular profiling, tissue imaging, and bioinformatics analysis. Core personnel
perform assays for investigators and provided a formalized training program to 45 trainees from
VDDRC Member laboratories on the theoretical and practical aspects of MS during the current funding
period. The small molecule/metabolite component of the Core is run as an open-access facility in which
users can choose to prepare their samples and operate the instruments. Proteomics samples are
submitted to the core for analysis by proteomics staff after consultations between the investigator and core
staff on the most appropriate experimental design, sample preparation and instrumental analysis methods.
Administrative staff monitor the use of the instrument facilities by investigators and prepare reports on
utilization for use by the Administrative Core. The core has 25 mass spectrometers available to users,
in addition to specialized instrumentation for advanced analyses. The Specific Aims of the Core are to:
1) Provide access to state-of-the-art mass spectrometry equipment for analysis of small molecule drugs
and endogenous metabolites (host and microbial), including targeted and discovery metabolomics, in an
open-access analytical facility; 2) Provide access to a state-of-the-art proteomics facility for identification,
characterization, and quantitation of proteins and whole proteomes in digestive disease-related samples;
3) Provide access to a pioneering imaging mass spectrometry facility for spatial localization of proteins,
peptides, lipids, metabolites, and drugs in GI tissues; 4) Provide professional guidance on experimental
design, development of analytical assays, and data interpretation; 5) Train users on digestive diseases
sample preparation, instrument operation, and data analysis; 6) Train users on bioinformatics analysis of
complex proteomic data; 7) Promote interactions ...

## Key facts

- **NIH application ID:** 10887471
- **Project number:** 5P30DK058404-23
- **Recipient organization:** VANDERBILT UNIVERSITY MEDICAL CENTER
- **Principal Investigator:** Kevin L Schey
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $79,182
- **Award type:** 5
- **Project period:** 2002-06-15 → 2027-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10887471

## Citation

> US National Institutes of Health, RePORTER application 10887471, Mass Spectrometry/Proteomics Core (5P30DK058404-23). Retrieved via AI Analytics 2026-06-14 from https://api.ai-analytics.org/grant/nih/10887471. Licensed CC0.

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