# Dissemination of MAPseq and BARseq for High-Throughput Brain Mapping

> **NIH NIH U24** · COLD SPRING HARBOR LABORATORY · 2024 · $929,890

## Abstract

The goal of this project is to disseminate MAPseq and BARseq to the broader neuroscience
community. These are novel methods developed in my laboratory based on high-throughput DNA
sequencing for determining neuronal circuitry. Neurons transmit information to distant brain
regions via long-range axonal projections. In some cases, functionally distinct populations of
neurons are intermingled within a small region. Disruptions of connectivity may underlie many
neuropsychiatric disorders including autism and schizophrenia.
At present, neuroanatomical techniques—particularly those with single neuron resolution—are
expensive and labor intensive. MAPseq and BARseq convert neuroanatomy into a problem of
DNA sequencing, and thereby allow us to exploit the tremendous breakthroughs in next-
generation sequencing throughput. The dissemination of these high-throughput methods for
determining neuronal projections will have important implications for understanding normal
neuronal circuitry, and how this circuitry is disrupted in animal models of neuropsychiatric
disorders like autism and schizophrenia.

## Key facts

- **NIH application ID:** 10888180
- **Project number:** 5U24NS126938-03
- **Recipient organization:** COLD SPRING HARBOR LABORATORY
- **Principal Investigator:** ANTHONY M ZADOR
- **Activity code:** U24 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $929,890
- **Award type:** 5
- **Project period:** 2022-08-01 → 2027-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10888180

## Citation

> US National Institutes of Health, RePORTER application 10888180, Dissemination of MAPseq and BARseq for High-Throughput Brain Mapping (5U24NS126938-03). Retrieved via AI Analytics 2026-06-11 from https://api.ai-analytics.org/grant/nih/10888180. Licensed CC0.

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