# Elucidation of the interactome for the bioactive disaccharide Man(B1-4)GlcNAc

> **NIH NIH R21** · SCRIPPS RESEARCH INSTITUTE, THE · 2024 · $271,500

## Abstract

PROJECT SUMMARY
Some autoimmune diseases, including systemic lupus erythematosus or “lupus,” are now known
to occur because of the pathogenic excess of an intracellular disaccharide called Man(b1-
4)GlcNAc. The accumulation of this disaccharide in cells can lead to the erroneous activation of
immune responses, leading to autoimmunity. The molecular mechanisms through which Man(b1-
4)GlcNAc regulate autoimmunity remain unknown, due to the difficulties of capturing the
intracellular interactors and receptors of free oligosaccharides, like Man(b1-4)GlcNAc. We will
use a chemoproteomic mass spectrometry-based approach to elucidate the interactome of
Man(b1-4)GlcNAc to identify its functional receptors. The accomplishments resulting from this
work will result in an enhanced understanding of aberrant glycosylation and the bioactivity of free
Man(b1-4)GlcNAc, as well as pave the pathway for studying the interactomes of intracellular free
oligosaccharides.

## Key facts

- **NIH application ID:** 10888732
- **Project number:** 1R21AI178437-01A1
- **Recipient organization:** SCRIPPS RESEARCH INSTITUTE, THE
- **Principal Investigator:** Mia L Huang
- **Activity code:** R21 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $271,500
- **Award type:** 1
- **Project period:** 2024-01-08 → 2025-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10888732

## Citation

> US National Institutes of Health, RePORTER application 10888732, Elucidation of the interactome for the bioactive disaccharide Man(B1-4)GlcNAc (1R21AI178437-01A1). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10888732. Licensed CC0.

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