# Streamline assessment of early lethal phenotypes in the mouse

> **NIH NIH R01** · UNIVERSITY OF MASSACHUSETTS AMHERST · 2024 · $602,413

## Abstract

PROJECT SUMMARY
Streamline assessment of early lethal phenotypes in the mouse
Although the generation of a loss of function allele at every locus in the mouse genome is well underway, there
is a gap in established pipelines for assessment of early lethal phenotypes (as stated in PAR-17-005). Here we
propose to continue our efforts to characterize up to 150 early lethal phenotypes occurring between fertilization
and organogenesis. As described within, we have instituted an efficient strategy to analyze early lethal
phenotypes and have already analyzed more than 100 novel phenotypes. We will provide a tremendous amount
of novel data to the scientific community and foster collaborative efforts towards functional annotation of the
mammalian genome. The proposed work capitalizes on techniques that our groups perform and publish
routinely, maximizing the data generation by eliminating training/troubleshooting steps as well as boosting our
individual research programs by providing novel phenotypes of interest. Characterization of knock-out alleles
will be invaluable towards understanding genetic pathways and predicting mechanisms of diseases/phenotypes
found in adults – in both heterozygotes and homozygous knockouts of genes in gene/protein networks or
pathways. We will provide detailed morphogenetic characterization for each mutant phenotype.
Characterization of novel gastrulation and preimplantation phenotypes will complement and extend our current
morphogenetic understanding of early developmental events.
Our proposal dovetails perfectly with existing phenotyping efforts and fills an essential need to characterize early
lethal phenotypes towards functional annotation of the genome.

## Key facts

- **NIH application ID:** 10889959
- **Project number:** 5R01HD083311-10
- **Recipient organization:** UNIVERSITY OF MASSACHUSETTS AMHERST
- **Principal Investigator:** Jesse Mager
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $602,413
- **Award type:** 5
- **Project period:** 2015-09-08 → 2025-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10889959

## Citation

> US National Institutes of Health, RePORTER application 10889959, Streamline assessment of early lethal phenotypes in the mouse (5R01HD083311-10). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10889959. Licensed CC0.

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