# Stanford MoTrPAC Site for Genomes, Epigenomes and Transcriptomes

> **NIH NIH U24** · STANFORD UNIVERSITY · 2024 · $885,999

## Abstract

PROJECT SUMMARY
Lack of physical activity is central to numerous health problems. Despite this, we have limited understanding of
the molecular components which confer the benefit of physical activity and their diverse impacts across tissues
with respect to age, sex, genetics, environment and other objective physiologic, morphometric, and metabolic
measures. Identifying these molecular signatures offers new opportunities for treatment and therapeutics. The
goal of the Molecular Transducers of Physical Activity in Humans Consortium (MoTrPAC) is to assemble a
comprehensive map of the molecular changes that occur in response to physical activity and, when possible,
relate these changes to the benefits of physical activity. This map will be greatly facilitated by the application of
–omics technologies to identify exercise-responsive genes and to relate their benefits across multiple tissues
and contexts. Our Stanford Chemical Analysis Site (CAS) is a core component of the MoTrPAC project.
Together with Mount Sinai School of Medicine, we form the Genome, Epigenomics and Transcriptomics (GET)
team. At Stanford CAS, we have provided molecular data on 7820 samples as part of both human and rat
components of MoTrPAC studies. We have further led the development of key quality control and data analysis
pipelines to ensure rigor and reproducibility. Our site has also led key consortium manuscripts and
engagement efforts from the animal training study. In the final phase of MoTrPAC, we principally aim to provide
a genome for every MoTrPAC participant and a transcriptome for every participant sample and facilitate
making this data broadly accessible and useful to the research community. Our site will be responsible for the
generation of 15784 transcriptomes using RNA-seq. In addition to this effort, we have proposed to generate
chromatin accessibility profiles using ATAC-seq on 1190 muscle and PBMC samples and long read
transcriptomes using Iso-Seq on 110 muscle samples. The expected benefits of ATAC-seq and Iso-seq efforts
will be to enable (1) mapping regulatory changes using chromatin accessibility data, (2) expand profiling of
genetic effects using allele-specific measurements and (3) provide complete isoform data for a reference
collection of MoTrPAC samples through exercise intervention. Our site provides critical prior expertise and
infrastructure to accomplish these production goals and analyses. We have demonstrated expertise for
analysis of collected tissues, compliance with MoTrPAC protocols, collaboration with other Chemical Analysis
Sites and all other aspects of the consortium, timely data deposition and support of the Bioinformatics Center
and expertise in data sharing, enhancement of rigor and reproducibility in Common Fund projects and leading
coordinated publication activities. Our PI team also provides MoTrPAC with important cross-consortia expertise
to extend its impacts and ensure timely completion. Overall, we are eager to complete the MoT...

## Key facts

- **NIH application ID:** 10893250
- **Project number:** 2U24DK112348-07
- **Recipient organization:** STANFORD UNIVERSITY
- **Principal Investigator:** Stephen Montgomery
- **Activity code:** U24 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $885,999
- **Award type:** 2
- **Project period:** 2016-12-13 → 2025-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10893250

## Citation

> US National Institutes of Health, RePORTER application 10893250, Stanford MoTrPAC Site for Genomes, Epigenomes and Transcriptomes (2U24DK112348-07). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10893250. Licensed CC0.

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