# Investigating mechanisms of cell volume regulation in the zebrafish notochord

> **NIH NIH R01** · DUKE UNIVERSITY · 2024 · $437,673

## Abstract

Abstract
 Animal cells achieve and maintain a typical volume through the regulation of ion channels that control osmotic
processes. In metazoans, development of properly proportioned bodies also requires volume regulation to be
coordinated amongst cells within tissues and across organs and systems. Although ion channels regulating cell
volume have been identified in cells in culture, little is known about the mechanisms that regulate cell volume
and coordinate volume control throughout whole tissues in vivo. Our proposed studies will investigate in zebrafish
a new and conserved pathway controlling cell volume and integrity in vacuolated cells of the notochord. Using
genetic, cell biological, physiological, and quantitative in vivo and ex vivo approaches, we will define the
physiological mechanisms regulating fluid transport and cell volume in response to mechanical cues.

## Key facts

- **NIH application ID:** 10893784
- **Project number:** 1R01GM150666-01A1
- **Recipient organization:** DUKE UNIVERSITY
- **Principal Investigator:** Michel Bagnat
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $437,673
- **Award type:** 1
- **Project period:** 2024-04-01 → 2028-02-29

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10893784

## Citation

> US National Institutes of Health, RePORTER application 10893784, Investigating mechanisms of cell volume regulation in the zebrafish notochord (1R01GM150666-01A1). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/10893784. Licensed CC0.

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