# The Jackson Laboratory Senescence Tissue Mapping Center (JAX-Sen TMC)

> **NIH NIH U54** · JACKSON LABORATORY · 2024 · $2,722,008

## Abstract

PROJECT SUMMARY/ABSTRACT OVERALL
The Jackson Laboratory (JAX) Cellular Senescence Network Mouse Tissue Mapping Center (JAX-Sen)
represents a multi-modal interdisciplinary effort that draws upon existing, longstanding aging research programs
at JAX, our role as a consortium site of KAPP-Sen, a funded SenNet human Tissue Mapping Center, and JAX’s
close collaboration with UConn Health. JAX-Sen is organized around three cores nested in the well-resourced
environment for aging research and multi-omics profiling at JAX. JAX-Sen will profile senescent cells in kidney,
adipose tissue, placenta, pancreas, heart, and hypothalamus, selected for their clinical relevance to chronic
diseases of aging, the domain expertise of the JAX-Sen leadership team in the biology of these tissues, and the
overlap of four of the tissues with KAPP-Sen. We will leverage cutting-edge mouse resources including the
Diversity Outbred, which offers unparalleled genetic diversity for modelling a range of molecular senescence
phenotypes, and new inbred (C57BL/6J) transgenic mice that express p16 and p21 driven fluorescent tags to
allow visualization enrichment for specific senescent cell subsets. The Biological Analysis Core will apply a
multitude of analytical modalities to selected tissues (bulk and single cell and single nucleus RNAseq, Visium
spatial transcriptomics, multiplexed antibody-based imaging, multiplexFISH, imaging mass spectrometry
including lipidomics, metabolomics, proteomics, 3D tissue reconstruction from serial sections), and will re-
evaluate these technologies throughout the project period to ensure our approaches align with those of other
centers and that we maximally benefit from the technology development efforts in SenNet. These profiling
activities will yield subcellular resolution of biomolecular content in senescent cells not possible with human
samples, along with an expanded set of senescent cell biomarkers. The Data Analysis Core will combine robust
computational capability with scalable and reproducible scientific workflows to develop and implement Network-
wide open data and metadata standards. The Data Analysis Core will benefit from JAX’s unique mouse research
resources to establish the diversity of senescence by integrating with expression quantitative trait locus and
protein quantitative trait locus data in DO mice and JAX bioinformatics resources, e.g., Mouse Genome
Informatics, Mouse Phenome Database, Monarch Initiative. The integrative mining of JAX-Sen data will lead to
novel methods for multi-modal 3D map reconstruction from imaging data and will yield an important resource for
SenNet interpretation and enrichment of human senescent cell data. Finally, the Administrative Core will provide
strong oversight of project progress and nurture a culture of collaborative, dynamic scientific exchange within
JAX-Sen. It will also maximize JAX-Sen’s engagement in the consortium through collaborative activities that will
help optimize data generation ac...

## Key facts

- **NIH application ID:** 10894253
- **Project number:** 5U54AG079753-03
- **Recipient organization:** JACKSON LABORATORY
- **Principal Investigator:** Ronny Korstanje
- **Activity code:** U54 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $2,722,008
- **Award type:** 5
- **Project period:** 2022-08-15 → 2026-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10894253

## Citation

> US National Institutes of Health, RePORTER application 10894253, The Jackson Laboratory Senescence Tissue Mapping Center (JAX-Sen TMC) (5U54AG079753-03). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10894253. Licensed CC0.

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