# Advanced Imaging Core

> **NIH NIH P01** · UNIVERSITY OF ILLINOIS AT CHICAGO · 2024 · $342,858

## Abstract

Abstract
Advanced Imaging Core C is essential to accurately define spatial and temporal organization of processes
governing macrophage plasticity. Surface markers determined by flow cytometry have been primarily used to
identify and sort diverse macrophage subpopulations. However, each Project makes clear that macrophage
diversity is fundamentally a consequence of specific spatiotemporal intracellular signaling and coordinated
transcriptional programming dictated by the niche environment. These processes control macrophage plasticity
during both inflammatory lung injury and its resolution. All Projects will address how inter-cellular and paracrine
signals interact with intracellular cues to alter macrophage fate in response to lung injury and resolution. Thus,
the function of Core C is to provide advanced reagents and imaging methodologies required for both in vitro and
in vivo studies and enable testing of hypotheses in each Project. We will provide the tools to address the
fundamental question that a given signaling pathway or transcriptional program is essential for the active
regulation of macrophage differentiation, specialization, and function. To gain the mechanistic signaling insights
needed for the success of each Project, Core C will provide methods at both conventional and super-resolution
levels to interrogate the signaling pathways in live macrophages. Spatiotemporal metabolic, transcriptional, and
signaling landscape changes in response to macrophage activation by ATP, β-catenin, CREB, STAT family
transcription factors and other key signals will be studied by multi-color super-resolution microscopy and super-
resolution activity imaging. These analyses of macrophages will permit a fine-grained assessment of the time-
sequence and spatial organization of the programs employed by macrophage variants. Although intravital
imaging is the most direct way to interrogate lung injury and recovery outcomes, respiratory motion in the live
animal represents a major obstacle for obtaining meaningful results by in vivo lung imaging. Thus, a major Core
C function will also be to provide advanced two-photon intravital lung imaging using a novel approach to reliably
identify myeloid cells in living mouse lung (in vessels and tissue) and track single cell motion, allowing each
Project to directly examine the functional effects of their pathway of interest during lung injury and repair phases.
By providing a seamless visual examination of signaling activity in macrophages as they differentiate, migrate,
and resolve immune challenges, Core C is essential for the Program’s overall success.

## Key facts

- **NIH application ID:** 10895337
- **Project number:** 5P01HL151327-04
- **Recipient organization:** UNIVERSITY OF ILLINOIS AT CHICAGO
- **Principal Investigator:** Gary CH Mo
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $342,858
- **Award type:** 5
- **Project period:** 2021-09-20 → 2026-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10895337

## Citation

> US National Institutes of Health, RePORTER application 10895337, Advanced Imaging Core (5P01HL151327-04). Retrieved via AI Analytics 2026-06-10 from https://api.ai-analytics.org/grant/nih/10895337. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*