# A Multi-scale Atlas of Senescence in Diverse Tissue Types

> **NIH NIH U54** · COLUMBIA UNIVERSITY HEALTH SCIENCES · 2024 · $2,587,409

## Abstract

OVERALL: PROJECT SUMMARY
Defining the molecular and cellular heterogeneity underlying senescent cell states is a critical knowledge gap in
the field. The Columbia University Senescence Tissue Mapping (CUSTMAP) Center is uniquely poised to
address this gap by creating a multi-scale atlas of senescence in diverse tissue types across the adult human
lifespan. CUSTMAP is a highly collaborative effort that builds upon long-standing and established collaborations
between Columbia University Irving Medical Center (CUIMC), The University of Edinburgh, New York University
(NYU), and the New York Genome Center (NYGC). Using state-of-the-art spatial genomics technologies and
leveraging our established experimental workflows and analytical pipelines, CUSTMAP will generate three-
dimensional maps of senescent cells in tissues with vulnerability to age-related degenerative processes: the
central nervous system (brain and spinal cord) and the skin. We will perform spatially resolved transcriptomics
(ST), single-nucleus RNA-sequencing, and multiplexed proteomics using iterative indirect immunofluorescence
imaging (4i) experiments in central nervous system (CNS) and skin tissues across the human lifespan, allowing
for unprecedented genome-wide molecular characterization at single-cell resolution in space. CUSTMAP is
structured around three scientific Cores, as well as an Administrative Core to support these integrated efforts.
Human tissue samples characterized and collected through the Biospecimen Core (BIO) will be analyzed using
the tools and techniques developed through the Biological Analysis Core (BAC) and Data Analysis Core (DAC),
leading to detailed maps of senescent cells and their effects in human tissues at single-cell resolution. The
success of CUSTMAP is predicated on access to a continuous supply of human tissues from healthy individuals
across the lifespan. CUSTMAP investigators have access to post-mortem samples and prospective tissue
collection from local resources at CUIMC as well as through established collaborations. Our unique geographic
location in Upper Manhattan enables tissue acquisition from a local population of patients that is rich in racial
and ethnic diversity. Thus, CUSTMAP is uniquely poised to obtain high-quality tissue from these diverse
populations and apply cutting-edge multiomic approaches to build integrated 3D molecular atlases of senescent
cells in CNS and skin tissues. Our approach provides a unique platform for driving transformative discoveries
of novel molecular, cellular and regional correlates of age-related changes in cellular senescence in human
tissues. The CUSTMAP workflow and computational tools are readily generalizable to other tissue types and
can be efficiently shared with and deployed across the SenNet Consortium.

## Key facts

- **NIH application ID:** 10898652
- **Project number:** 5U54AG076040-04
- **Recipient organization:** COLUMBIA UNIVERSITY HEALTH SCIENCES
- **Principal Investigator:** Angela M Christiano
- **Activity code:** U54 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $2,587,409
- **Award type:** 5
- **Project period:** 2021-09-30 → 2026-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10898652

## Citation

> US National Institutes of Health, RePORTER application 10898652, A Multi-scale Atlas of Senescence in Diverse Tissue Types (5U54AG076040-04). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10898652. Licensed CC0.

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