Project Summary The New York State Department of Health (NYSDOH) Wadsworth Center (WC) public health laboratory is committed to protecting and improving the health of New Yorkers through laboratory testing analysis, investigations and research. The specific aim of this project will be to improve detection of, and surveillance for, antibiotic resistance for Salmonella, Campylobacter, Enterococcus, Aeromonas, Vibrio and other lactose positive bacteria isolated from food products purchased from selected grocery stores. Objectives will be to: 1) collect and test samples for Salmonella, Campylobacter, Enterococcus, Aeromonas, Vibrio and other lactose positive bacteria utilizing microbiological tests; 2) collect, analyze and exchange surveillance data on isolate identifications and provide whole genome sequence data and serotyping analysis; 3) ship isolates attained from meat and seafood samples for additional testing to FDA; and 4) collaborate with FDA, Center for Veterinary Medicine (CVM), NARMS and state laboratory partners. Outcomes expected from this work include: 1) providing an increase in the total amount of Salmonella, Campylobacter Enterococcus, Aeromonas, Vibrio and other lactose positive bacteria isolates from retail meats and seafood that can be analyzed; 2) maintaining the ability to compare results to surveillance data and exchange information and test results; 3) providing an increase in the number of isolates submitted to the FDA; and 4) increased collaboration and communication among partnering entities.