# Experimental Mouse Resources Core

> **NIH NIH U54** · INDIANA UNIVERSITY INDIANAPOLIS · 2024 · $190,835

## Abstract

The Cooperative Centers of Excellence in Hematology (CCEH) will be utilized by investigators for in vivo
studies focused on molecular and cellular mechanisms involved in steady state hematopoiesis, the bone
marrow niche, BM transplantation, and immune cell function. Centralization will prevent duplication of
resources, ensure consistent supply of acclimated mice, and maintain high quality research infrastructure
essential for conducting in vivo studies of hematopoietic stem and progenitor cell (HSPC) function. The
Experimental Mouse Resources Core (EMRC) will provide CCEH investigators advanced resources for their
studies using mice for murine and human transplantation models. The specific aims of the EMRC are:
1. Maintain on-site breeding colonies of essential mice to study human and murine hematopoiesis in
 vivo. For investigations focused on human hematopoiesis, colonies of NOD.Cg-PrkdcscidIl2rgtm1Wjl/J (NSG)
 and NOD.Cg-Ragtm1MomIl2rgtm1Wjl/SzJ and (NRG) immunodeficient mice will be maintained. In anticipation of
 future needs, a starter colony of the triple transgenic NSG-SGM3 (NSGS) mice that express human IL3,
 GM-CSF and SCF has been established and can be expanded as needed. Breeding colonies of
 C57/BL/6J, B6.SJL-Ptprca Pepcb/BoyJ (Boy/J), and the BoyJ x C57BL/6J (F1 cross) mouse strains will be
 maintained. The F1 cross is a unique strain and is not commercially available. Congenic mouse strains
 allow for simultaneous tracking of donor, competitor, and recipient cells.
2. Provide specialized core services and consultation to CCEH investigators. EMRC personnel will
 conduct irradiations, transplantations, serial sampling of peripheral blood, and compound dosing. The Core
 will harvest and provide tissues from transplanted mice to investigators for detailed analysis by the CCEH
 Flow and Tissue Cytometry Core. The ERMC will work closely with the Hypoxia Core to coordinate
 transplant of mouse and human HSPC collected and processed under hypoxia versus ambient air (Mantel
 et al., Cell 2015). The EMRC Director will coordinate with Core personnel all procedures, experimental
 design, methodology, and data interpretation. Expertise and infrastructure is in place to facilitate all aspects
 of BMT studies seamlessly for on-site CCEH members as well as PIs on a national level.
3. Serve as an educational resource for CCEH laboratories. Exceptional connectivity between the EMRC
 and the other biomedical cores as well as the Enrichment core will augment the research and training
 activities of the CCEH membership and their trainees. Training in the execution of high-quality in vivo
 studies will be a critical and essential component in training our next generation of scientists to be
 successful in research careers dedicated to the study of nonmalignant hematology.
4. Work in concert with other CCEH centers and investigators Nationwide to provide EMRC services to
 the community of scientists involved in nonmalignant hematology research.

## Key facts

- **NIH application ID:** 10899547
- **Project number:** 5U54DK106846-09
- **Recipient organization:** INDIANA UNIVERSITY INDIANAPOLIS
- **Principal Investigator:** Karen Elizabeth Pollok
- **Activity code:** U54 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $190,835
- **Award type:** 5
- **Project period:** 2015-08-01 → 2026-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10899547

## Citation

> US National Institutes of Health, RePORTER application 10899547, Experimental Mouse Resources Core (5U54DK106846-09). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10899547. Licensed CC0.

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