Abstract Chemotherapy treatment frequently results in taste alterations that significantly impact patients' quality of life and recovery. While transient loss of taste receptor cells (TRCs) has been observed in mice after chemotherapy, this does not fully explain the prolonged taste disruptions reported by human patients. My preliminary data indicates that gustatory fiber innervation in taste buds is reduced shortly after chemotherapy, but the recovery of these connections and their effects on taste response remain unclear. This study aims to investigate the timeline of gustatory fiber recovery after cyclophosphamide (CYP) treatment and assess the strength and specificity changes in taste signaling. To determine the time course of gustatory fiber reinnervation within taste buds after CYP treatment, I will use immunohistochemistry and intravital imaging to monitor the recovery of gustatory fibers and taste synapses over 20+ days post-treatment. To assess the degree to which cyclophosphamide alters the strength and specificity of taste signaling in mice, I will use whole-nerve recording and in-vivo calcium imaging. This proposal is designed to unravel the recovery process of gustatory fiber innervation after chemotherapy and explore strength and specificity changes in taste signaling. In addition, activities planned for this proposal will strengthen my career development and training to achieve my goal of becoming a leader in investigating neuro-immune interactions.