# Lipoprotein Quantitation and Function Core

> **NIH NIH P01** · UNIVERSITY OF CINCINNATI · 2024 · $313,438

## Abstract

ABSTRACT (Core C)
High quality, consistent and reproducible quantitation and functional data are critical for the success of this
Program Project. The overarching goal of the HDL Quantitation and Function Core (Core C) is to meet this
need in a centralized and consistent manner across the entire Program. The Core is structured to provide the
following services: 1) quantitation of HDL particle size and concentration (whether isolated from plasma or
reconstituted HDL particles) for specific structural studies, using calibrated differential ion mobility analysis
(cIMA); 2) mass spectrometric analyses to characterize the protein composition of HDL particles isolated or
prepared by Program investigators, and chemical crosslinking strategies to support their structural studies; the
Core will also use mass spectrometry to confirm the identity and purity of the recombinant proteins prepared by
Core D; 3) a combination of accurate measurement of HDL particle concentration by calibrated IMA and
accurate quantification of apolipoproteins by LC-MS/MS, including quantification of the stoichiometry of
apolipoproteins on HDL particles; 4) bioinformatic support for interpreting HDL-P and proteomic data sets and
for combining the data with information from Gene Ontology, protein-protein interactions, and pathway
analysis; 5) measurements of cholesterol and phospholipid efflux, using baby hamster kidney cells
conditionally expressing WT and mutant human ABCA1. Core C will be led by Tomas Vaisar, Ph.D., working
closely with Chongren Tang, Ph.D., and it will be located at the University of Washington South Lake Union
campus, where Project 1 is also located. Both labs have extensive, well-documented expertise in all areas of
the Core’s proposed services. In the previous PPG cycle, this Core performed over 1000 analyses of HDL-P
and size, over 500 LC-MS/MS analyses of HDL’s proteome, and crosslinking studies for characterizing and
experimentally verifying theoretical models of HDL and ABCA1 structures. These services will be extended in
this proposal. Collectively, they will enable cost-effective and rigorous testing of the hypotheses generated by
the various Projects.

## Key facts

- **NIH application ID:** 10903858
- **Project number:** 5P01HL128203-07
- **Recipient organization:** UNIVERSITY OF CINCINNATI
- **Principal Investigator:** Tomas Vaisar
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $313,438
- **Award type:** 5
- **Project period:** 2016-09-15 → 2028-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10903858

## Citation

> US National Institutes of Health, RePORTER application 10903858, Lipoprotein Quantitation and Function Core (5P01HL128203-07). Retrieved via AI Analytics 2026-06-01 from https://api.ai-analytics.org/grant/nih/10903858. Licensed CC0.

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