# Apo/Lipoprotein Production Core

> **NIH NIH P01** · UNIVERSITY OF CINCINNATI · 2024 · $251,997

## Abstract

SUMMARY (Core D)
The multidisciplinary approaches proposed in this Program Project, whether structural or functional, depend on
the availability of high quality and standardized biological samples and reagents. The overarching goal of the
Apo/Lipoprotein and Production Core (Core D) is to meet this critical need across the entire Program. The
Core is designed to provide the following services: 1) Isolation, characterization, and distribution of lipoproteins
and apolipoproteins isolated from human plasma, 2) Expression, purification, and distribution of recombinant
apolipoproteins, isotopically labeled proteins, and peptide mass spectrometry standards, 3) Design,
expression, purification, and distribution of customized apolipoprotein mutants, including new constructs for
assessing the mobility of HDL-bound proteins, 4) Generation, characterization, and distribution of reconstituted
HDL particles customized to test specific hypotheses from around the Program, and 5) Generation and
characterization of targeted nanobody reagents for cryo-EM studies. Core D will be led by W. Sean Davidson,
Ph.D., working with two outstanding and highly experienced research technicians, Jamie Morris and Scott
Street. It will be located at the University of Cincinnati, which has extensive and documented experience in all
of the Core’s proposed services. In the previous PPG cycle, Core D filled over 550 orders from the other
Projects and Cores, not including hundreds more that were used in the Davidson lab. Based on prices charged
by traditional biochemical supply sources, the retail value of these samples far exceeds the resources needed
to run the core. Thus, this resource contributes substantially to the synergy and cost-effectiveness of the
Program’s ongoing work. We believe that few, if any, facilities in the world can provide these services in a
single place.

## Key facts

- **NIH application ID:** 10903860
- **Project number:** 5P01HL128203-07
- **Recipient organization:** UNIVERSITY OF CINCINNATI
- **Principal Investigator:** W Sean Davidson
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $251,997
- **Award type:** 5
- **Project period:** 2016-09-15 → 2028-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10903860

## Citation

> US National Institutes of Health, RePORTER application 10903860, Apo/Lipoprotein Production Core (5P01HL128203-07). Retrieved via AI Analytics 2026-05-29 from https://api.ai-analytics.org/grant/nih/10903860. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*