# The Biology of Peroxiredoxin 6

> **NIH NIH R35** · UNIVERSITY OF CALIFORNIA BERKELEY · 2024 · $20,491

## Abstract

Project Summary/Abstract
Peroxiredoxin 6 (Prdx6) is a multi-functional enzyme that expresses glutathione peroxidase, phospholipase A2,
and lysophosphatidylcholine acyltransferase activities in separate catalytic sites. Prdx6 can reduce
phospholipid hydroperoxides and hydrolyze and re-acylate phospholipid fatty acyl bonds. Prdx6 is, therefore, a
complete enzyme for the repair of peroxidized cell membranes. Our lab studies the contributions of the
different catalytic activities of Prdx6 to the suppression of ferroptosis, an iron-dependent form of regulated cell
death driven by the accumulation of phospholipid hydroperoxides. Ferroptosis is implicated in
ischemia/reperfusion (I/R) injury, but the role of Prdx6 in this process is unknown. Here, we propose studying
how Prdx6 regulates ferroptosis in cellular renal I/R models. Renal I/R is the most prevalent cause of acute
kidney injury, a clinically impactful disease that significantly affects patient management regarding the
treatment options for their primary disease. We will use flow-adapted endothelial cells and kidney organoids
derived from Prdx6 KO and KI mice carrying single point mutations that inactivate each of the Prdx6 activities
alone to study how Prdx6 regulates oxidant stress, ferroptosis, and inflammatory signaling in renal I/R while
providing postdoctoral training to Dr. Justin Conner who seeks to expand his skills and become a competitive
candidate for a MOSIAC K99/R00 application in next two years, with the ultimate goal to become an
independent biomedical investigator.

## Key facts

- **NIH application ID:** 10906525
- **Project number:** 3R35GM146951-02S1
- **Recipient organization:** UNIVERSITY OF CALIFORNIA BERKELEY
- **Principal Investigator:** Jose P Vazquez Medina
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $20,491
- **Award type:** 3
- **Project period:** 2022-09-01 → 2027-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10906525

## Citation

> US National Institutes of Health, RePORTER application 10906525, The Biology of Peroxiredoxin 6 (3R35GM146951-02S1). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10906525. Licensed CC0.

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