# Blood Flow Regulation and Neuromuscular Function Post-Stroke > **NIH NIH R01** · MEDICAL COLLEGE OF WISCONSIN · 2024 · $641,044 ## Abstract Increased neuromuscular fatigability (the acute, exercise induced reduction in force) is an understudied consequence of stroke. This is a clinically meaningful area of study because increased neuromuscular fatigability can negatively affect task endurance for activities like walking, and successful rehabilitation strategies require repeated levels of muscle activation and overload to cause functional gains in motor performance. In addition to decreased neural drive to motorneuron pools, recent data indicate that reduced blood flow to exercising paretic muscle may play a significant role in increased neuromuscular fatigability. Exercising muscles require adequate blood flow to match the increase in metabolic demand, and we have shown that stroke survivors have reduced blood flow to the leg muscles during exercise. During exercise, sympathetic nervous system activity increases in an activity-dependent manner, causing vasoconstriction in inactive muscle beds. In the active muscle, the release of local vasodilatory factors counteracts sympathetic vasoconstriction to maintain vascular tone. This process, called functional sympatholysis, has been postulated to be critical to muscle perfusion during exercise. Our central hypothesis is that in people with stroke functional sympatholysis is impaired and results in dysregulated blood flow during exercise, which exacerbates neuromuscular fatigability and limits motor function. We propose three specific aims. In Aim 1 we will establish impaired functional sympatholysis in chronic stroke survivors and determine the relationship with metrics of neuromuscular fatigue. We will test two hypotheses in Aim 1: 1) that functional sympatholysis during exercise is impaired in the paretic leg of chronic stroke survivors compared to the non-paretic leg and age- and sex-matched controls, and 2) stroke survivors with the highest degree of functional sympatholysis impairment will have greater paretic leg muscle fatigability, and both impaired modulation of motor unit firing rates and increased metabolite buildup in the muscle during exercise. In Aim 2 we will interrogate microvascular (dys)function in the lower extremity of chronic stroke survivors. We will test two hypotheses in Aim 2: 1) that compared age- and sex-matched controls, chronic stroke survivors will have reduced nitric oxide-mediated vasodilation to acetylcholine and an enhanced vasoconstrictor response to locally infused norepinephrine, and 2) that maximum dilation to acetylcholine in the affected leg will be positively associated with lower paretic muscle fatigability. Finally, in Aim 3 we will determine if a non-invasive intervention called ischemic conditioning (IC), which is known to improve muscle performance and vascular endothelial function, can improve functional sympatholysis, and if improvements in functional sympatholysis are associated with reduced paretic muscle fatigability. We will test two hypotheses in Aim 3: 1) that IC causes immediate and s... ## Key facts - **NIH application ID:** 10906947 - **Project number:** 5R01HD112258-02 - **Recipient organization:** MEDICAL COLLEGE OF WISCONSIN - **Principal Investigator:** Matthew J Durand - **Activity code:** R01 (R01, R21, SBIR, etc.) - **Funding institute:** NIH - **Fiscal year:** 2024 - **Award amount:** $641,044 - **Award type:** 5 - **Project period:** 2023-08-15 → 2028-07-31 ## Primary source NIH RePORTER: https://reporter.nih.gov/project-details/10906947 ## Citation > US National Institutes of Health, RePORTER application 10906947, Blood Flow Regulation and Neuromuscular Function Post-Stroke (5R01HD112258-02). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/10906947. Licensed CC0. --- *[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*