# Deciphering long-term virus evolution through the reconstruction of past viral genomes

> **NIH NIH DP2** · FRED HUTCHINSON CANCER CENTER · 2024 · $508,951

## Abstract

Project Summary
Viruses have imposed a significant global burden of morbidity and mortality for millennia. Just in the last
century, highly pathogenic RNA viruses such as influenza A, HIV and SARS-CoV-2 resulted in devastating
pandemics that directly impacted millions of individuals and altered socioeconomic dynamics worldwide.
Fueled by major advances in sequencing during the last decades, the emerging field of archeovirology has
begun identifying viruses that severely impacted humans prior to the 20th century. To this day, however, the
pathogens responsible for important past epidemics are still unknown and there are significant gaps remaining
in the evolutionary history of certain viral families, especially for RNA viruses. Using a highly collaborative and
innovative approach to overcome intrinsic limitations in the identification of viral genomes from human remains,
this proposal will uncover the viral diversity that existed during periods of notable epidemic outbreaks and
expand our knowledge of the origins and evolution of highly pathogenic viruses. In particular, this proposal
seeks to identify and characterize genomes from centuries-old RNA viruses, a goal that so far has remained
elusive. Using well-established ancient DNA techniques, forensic proteomics, and improved RNA isolation
methodologies, our team will reconstruct viral genomes from human remains of deadly epidemics in early
colonial Mexico and preserved lung specimens from pathology collections corresponding to the industrial
revolution in Great Britain. Guided by archeological and historical documentation in these two distinct historical
contexts, this work will identify viruses that existed in the past ~500 years, study their origin and evolutionary
relationships to modern viruses, and characterize the evolution of viral protein function in relation to their
human hosts. Together, this proposal will generate a comprehensive characterization of the properties of past
viral infections that will not only lead to the identification of viruses responsible for epidemics that profoundly
altered human history but will also uncover evolutionary adaptations between past and present viruses and
define the origins of highly pathogenic RNA viruses, providing key molecular information to prepare against the
(re)emergence of highly virulent viruses in the future.

## Key facts

- **NIH application ID:** 10909332
- **Project number:** 5DP2AI177896-02
- **Recipient organization:** FRED HUTCHINSON CANCER CENTER
- **Principal Investigator:** Daniel Blanco-Melo
- **Activity code:** DP2 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $508,951
- **Award type:** 5
- **Project period:** 2023-08-17 → 2028-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10909332

## Citation

> US National Institutes of Health, RePORTER application 10909332, Deciphering long-term virus evolution through the reconstruction of past viral genomes (5DP2AI177896-02). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10909332. Licensed CC0.

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