# A comprehensive binding and functional map of human RNA-binding proteins

> **NIH NIH U24** · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · 2024 · $1,345,922

## Abstract

Project Summary
The objective of the ENCORE (Encyclopedia of RNA Elements) project is to develop a foundational,
functional map of protein-RNA interactions of RNA binding proteins (RBPs) encoded in the human genome,
and the RNA elements they bind to across the transcriptome. These RNA elements, when expressed, form
the basis of co- and post-transcriptional regulation of human genes. Our strategy consists of developing and
integrating a physical map of 400 new RBPs in two different human cell lines with transcriptome-wide
measurements of the effects of depleting these RBPs. Over the past 9 years, our group has established highly
efficient data production workflows of experimental methods that will enable us to immediately expand these
datasets further, which form a crucial and missing link to decipher the mechanisms of post-trancriptional
regulation and how these impact genetic variation and disease etiology. When combined with the data we
generated over the past 9 years, these efforts will culminate in a comprehensive map of the functional RNA
elements recognized by essentially all RBPs expressed in two human cell lines, representing approximately
half of the known complement of human RBPs. ENCORE will continue developing and integrating a physical
map of hundreds of RBPs in two different human cell lines with transcriptome-wide measurements of the
effects of depleting these RBPs. In addition, we will provide training and outreach to establish ENCORE
annotations as the standard reference for co- and post-transcriptional research and clinical genomics efforts
in the long-term. In summary, the data we will produce in this project will enable a more systematic and
comprehensive understanding of the role of RBPs and RNA biology in the contribution to human biology and
disease.

## Key facts

- **NIH application ID:** 10909889
- **Project number:** 5U24HG009889-07
- **Recipient organization:** UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
- **Principal Investigator:** Brenton R. Graveley
- **Activity code:** U24 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $1,345,922
- **Award type:** 5
- **Project period:** 2018-02-05 → 2026-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10909889

## Citation

> US National Institutes of Health, RePORTER application 10909889, A comprehensive binding and functional map of human RNA-binding proteins (5U24HG009889-07). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10909889. Licensed CC0.

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